Analysis of HLA-A24-restricted CMVpp65 peptide-specific CTL with HLA-A*2402-CMVpp65 tetramer
Analysis of HLA-A24-restricted CMVpp65 peptide-specific CTL with HLA-A*2402-CMVpp65 tetramer
复制标题
DOI：
10.1016/j.imlet.2004.07.010
复制
发表时间：
2004-09-01
影响因子：
4.4
通讯作者：
Yamaguchi, K
中科院分区：
文献类型：
--
作者：
Akiyama, Y;Kuzushima, K;Yamaguchi, K
发展精确和有效的方法来监测免疫功能低下的移植患者对巨细胞病毒（CMV）感染的免疫应答是非常重要的。为了优化监测策略，对CMVpp 65肽特异性CTL系进行了基于HLA-A24-CMVpp 65四聚体的分析。先前，鉴定了CMVpp 65基质蛋白的HLA-A24限制性CTL表位（QYDPVAALF aa 341-349）。本研究采用肽脉冲树突状细胞（DC）对12例HLA-A24（+）健康人外周血淋巴细胞进行两次刺激，获得CMVpp 65（aa 341-349）肽特异性CTL细胞株。在12株CTL细胞中，9株表现为HLA-A*2402-CMVpp 65四聚体染色，并与CMVpp 65肽估计的CTL细胞产生IFN-γ的量密切相关（r = 0.943，P < 0.001）。提示HLA-A*2402-CMVpp 65四聚体染色是监测HLA-A24（+）免疫受损宿主抗CMV感染免疫应答的有效方法。(C)2004 Elsevier B. V.保留所有权利。
Developing precise and efficient methods of monitoring immune responses against cytomegalovirus (CMV) infection in immunocompromised transplantation patients is important. With the aim of optimizing the monitoring strategy, an HLA-A24-CMVpp65 tetramer-based analysis of CMVpp65 peptide-specific CTL lines was performed. Previously, the HLA-A24-restricted CTL epitope of CMVpp65 matrix protein was identified (QYDPVAALF aa 341-349). In the present study, CMVpp65 (aa 341-349) peptide- specific CTL lines were obtained from PBLs of 12 HLA-A24(+) healthy donors by two stimulations with peptide-pulsed dendritic cells (DC). Among 12 CTL lines, 9 showed HLA-A*2402-CMVpp65 tetramer staining, which was found to be strongly co-related to the amount of IFN-gamma produced by CMVpp65 peptider-estimulated CTL lines (r = 0.943, P < 0.001). These results suggested that HLA-A*2402-CMVpp65 tetramer staining was an efficient way to monitor immune responses against CMV infection in HLA-A24(+) immunocompromised hosts. (C) 2004 Elsevier B.V. All rights reserved.