Lipase-Catalyzed Concentration of Stearidonic Acid in Modified Soybean Oil by Partial Hydrolysis
Lipase-Catalyzed Concentration of Stearidonic Acid in Modified Soybean Oil by Partial Hydrolysis
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DOI：
10.1007/s11746-012-2108-9
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发表时间：
2012-07
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影响因子：
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通讯作者：
Leslie Kleiner;L. Vázquez;C. Akoh
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文献类型：
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作者：
Leslie Kleiner;L. Vázquez;C. Akoh
脂质酸（SDA, 18:4 ω-3）的改性大豆油（MSO）含有~ 25%的SDA，通过脂肪酶催化水解提高了其含量。四种非固定化粉状脂肪酶，脂肪酶ay30（白色念珠菌）、脂肪酶g50 （camembertii青霉）、Lipomod™34P-L034P（白茅念珠菌）、Lipomod™36P-L036P（米根霉）和固定化脂肪酶Lipozyme RM（米黑根霉），在不同的培养时间内，评估了它们水解MSO的能力和对SDA的特异性。SDA富集产物含有三酰基甘油（TAG）、二酰基甘油（DAG）和单酰基甘油（MAG）。脂肪酶34P-L034P对SDA具有特异性，而脂肪酶AY对SDA具有特异性。天野AY脂肪酶在培养4 h时获得最高的总SDA含量（40.9 mol%）（水解率为66.2%）。未水解TAG、1,3-DAG、2,3(1)-DAG和MAG的SDA含量分别为37.7% （sn-2位置为56.4）、41.6、51.5 （sn-2位置为54.9%）和49.9%。Amano AY脂肪酶也用于水解先前从MSO低温结晶中获得的富含SDA的TAG （48.7% SDA）。在孵育12 h（85.9%水解）时获得最高的总SDA含量（62.7 mol%）。未水解TAG、1,3-DAG、2,3(1)-DAG和MAG的SDA含量分别为58.7% （sn-2位置为65.7）、71.2、70.2 （sn-2位置为52.9）和59.4%。
Stearidonic acid (SDA, 18:4 ω-3) content of modified soybean oil (MSO) containing ~25 % SDA, was increased by lipase-catalyzed hydrolysis. Four non-immobilized powdered lipases, Lipase AY 30 (Candida rugosa), Lipase G 50 (Penicillium camembertii), Lipomod™ 34P-L034P (Candida cylindracea [rugosa]), Lipomod™ 36P-L036P (Rhizopus oryzae), and an immobilized lipase, Lipozyme RM IM (Rhizomucor miehei) were assessed, at various incubation times, for their ability to hydrolyze MSO and specificity toward SDA. The SDA enriched products contained triacylglycerols (TAG), diacylglycerols (DAG) and monoacylglycerols (MAG). Lipase 34P-L034P exhibited specificity towards SDA, while Lipase AY was able to discriminate against it. The highest total SDA content (40.9 mol%) was obtained with Amano AY lipase at 4 h incubation (66.2 % hydrolysis). Unhydrolyzed TAG, 1,3-DAG, 2,3(1)-DAG, and MAG contained 37.7 (56.4 at the sn-2 position), 41.6, 51.5 (54.9 at the sn-2 position), and 49.9 % SDA, respectively. Amano AY lipase was also used to hydrolyze previously SDA-enriched TAG (48.7 % SDA) obtained from low temperature crystallization of MSO. The highest total SDA content (62.7 mol%) was obtained at 12 h incubation (85.9 % hydrolysis). The SDA contents of unhydrolyzed TAG, 1,3-DAG, 2,3(1)-DAG, and MAG were 58.7 (65.7 at the sn-2 position), 71.2, 70.2 (52.9 at the sn-2 position), and 59.4 %, respectively.