A polychaete hunchback ortholog.
A polychaete hunchback ortholog.
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DOI：
10.1006/dbio.2001.0272
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发表时间：
2001-07
影响因子：
2.7
通讯作者：
A. H. Werbrock;D. A. Meiklejohn;A. Sainz;J. H. Iwasa;R. M. Savage
中科院分区：
文献类型：
--
作者：
A. H. Werbrock;D. A. Meiklejohn;A. Sainz;J. H. Iwasa;R. M. Savage
我们报告的第一个特征的分割基因同源性在基础多毛类Capitella capitata使用泛环节动物跨物种抗体的驼背样基因产物。在苍蝇中，差距分割基因hunchback（hb）编码一种C（2）H（2）锌指转录因子，该因子在苍蝇身体平面前部区域的图案化中发挥着关键作用。在Capitella（Cc-hb）中的hb直向同源物在整个卵裂过程中表达于母体和所有的小单体和大单体细胞中。在原肠胚形成时，核Cc-hb蛋白在经历外生的微粒衍生的表面上皮和逐渐内化的大植物性卵裂球中表达。在器官形成过程中，Cc-hb在发育中的肠上皮、前列腺和臀管上皮以及成人中枢神经系统中分化的神经元的子集中表达。Cc-hb在躯干的节段性前体细胞中不表达。在Capitella中的Cc-hb表达结构域与水蛭hb直系同源物（LZF 2）的报道相似，并且许多观察到的环节动物类之间的差异与生活史的变化相关。在水蛭和多毛类动物的AP模式形成时，躯干中缺乏可检测到的环节动物血红蛋白，这表明果蝇中血红蛋白的前组织功能起源于节肢动物或昆虫谱系。
We report the first characterization of a segmentation gene homologue in the basal polychaete Capitella capitata using a pan-annelid cross-species antibody to the hunchback-like gene product. In flies, the gap segmentation gene hunchback (hb) encodes a C(2)H(2) zinc-finger transcription factor that plays a pivotal role in patterning the anterior region of the fly body plan. The hb orthologue in Capitella (Cc-hb) is expressed maternally and in all micromere and macromere cells throughout cleavage. At gastrulation, nuclear Cc-hb protein is expressed in the micromere-derived surface epithelium that undergoes epiboly and in the large vegetal blastomeres that gradually become internalized. During organogenesis, Cc-hb is expressed in the developing gut epithelium, the prostomial and pygidial epithelium, and in a subset of differentiated neurons in the adult central nervous system. Cc-hb is not expressed in the segmental precursor cells in the trunk. The Cc-hb expression domains in Capitella are similar to those reported for the leech hb orthologue (LZF2), and many of the observed differences between the annelid classes correlate with changes in life history. The lack of detectable annelid hb protein in the trunk at the time of AP pattern formation in leech and in polychaete suggests that the anterior organizing function of hb in flies originated in the arthropod or insect lineage.