Geometric relationship between the nicotinamide and isoalloxazine rings in NADPH-cytochrome P-450 oxidoreductase: implications for the classification of evolutionarily and functionally related flavoproteins.
Geometric relationship between the nicotinamide and isoalloxazine rings in NADPH-cytochrome P-450 oxidoreductase: implications for the classification of evolutionarily and functionally related flavoproteins.
复制标题
NADPH-细胞色素 P-450 氧化还原酶中烟酰胺和异咯嗪环之间的几何关系：对进化和功能相关黄素蛋白分类的影响。
DOI：
10.1021/bi00128a013
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发表时间：
1992
期刊：
影响因子：
2.9
通讯作者：
Kasper,CB
中科院分区：
文献类型：
--
作者：
Sem,DS;Kasper,CB
1992年1月23日收到的修订版Mandarin pt摘要：已确定黄素蛋白NADPH-细胞色素P-450氧化还原酶（P-450 R）从NADPH中提取氢化物的立体特异性和糖苷键周围烟酰胺环的构象。A侧（pro-R）氢从NADPH中提取，烟酰胺环处于反构象。这些结果与A侧立体特异性与反构象之间以及B侧立体特异性与顺式构象之间明显的强相关性一致[You，K.（1985）CRC Crit.Rev.Biochem.17，313]。这种相关性揭示了黄素和烟酰胺环是如何相对于彼此定向的。在P-450 R中，黄素然后“在
Revised Manuscript Received January 23, 1992 abstract: The stereospecificity of hydride abstraction from NADPH and the conformation of the nico-tinamide ring around the glycosidic bond have been determined for the flavoprotein NADPH-cytochrome P-450 oxidoreductase (P-450R). The A-side (pro-R) hydrogen is abstracted from NADPH, and the nicotinamide ring is in the anti conformation. These results are consistent with the apparently strong correlation between A-side stereospecificity and anti conformation and between B-side stereospecificity and syn conformation [You, K.(1985) CRC Crit. Rev. Biochem. 17, 313]. This correlation reveals how the flavin and nicotinamide rings are oriented relative to each other. In P-450R, the flavin is then “on top of