Circulating MicroRNA-208b and MicroRNA-499 Reflect Myocardial Damage in Cardiovascular Disease
Circulating MicroRNA-208b and MicroRNA-499 Reflect Myocardial Damage in Cardiovascular Disease
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DOI：
10.1161/circgenetics.110.957415
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发表时间：
2010-12-01
影响因子：
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通讯作者：
Schroen, Blanche
中科院分区：
文献类型：
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作者：
Corsten, Maarten F.;Dennert, Robert;Schroen, Blanche
背景-被称为microRNAs的小RNA分子在人的血浆中自由循环，并与各种病理相关。在这项研究中，我们探索了它们在一些常见心血管疾病中的诊断潜力。方法和结果-从具有不同程度心脏损害的患者的血浆中提取microRNAs：(1)急性心肌梗死，(2)病毒性心肌炎，(3)舒张期功能障碍，(4)急性心力衰竭。随后用实时聚合酶链式反应检测选定的microRNAs的血浆水平，包括心脏相关的(miR-1、-133a、-208b和-499)、纤维化相关的(miR-21和miR-29b)和白细胞相关的(miR-146、-155和-223)。在急性心肌梗死患者血浆中，心肌细胞相关miR-208B和-499显著升高，是对照组的1600倍(P
Background-Small RNA molecules, called microRNAs, freely circulate in human plasma and correlate with varying pathologies. In this study, we explored their diagnostic potential in a selection of prevalent cardiovascular disorders.Methods and Results-MicroRNAs were isolated from plasmas from well-characterized patients with varying degrees of cardiac damage: (1) acute myocardial infarction, (2) viral myocarditis, (3) diastolic dysfunction, and (4) acute heart failure. Plasma levels of selected microRNAs, including heart-associated (miR-1, -133a, -208b, and -499), fibrosis-associated (miR-21 and miR-29b), and leukocyte-associated (miR-146, -155, and -223) candidates, were subsequently assessed using real-time polymerase chain reaction. Strikingly, in plasma from acute myocardial infarction patients, cardiac myocyte-associated miR-208b and -499 were highly elevated, 1600-fold (P