Study of an electroosmotic pump for liquid delivery and its application in capillary column liquid chromatography
Study of an electroosmotic pump for liquid delivery and its application in capillary column liquid chromatography
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DOI：
10.1016/j.chroma.2003.11.071
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发表时间：
2004-03-05
影响因子：
4.1
通讯作者：
Guan, YF
中科院分区：
文献类型：
--
作者：
Chen, LX;Ma, JP;Guan, YF
构建了一种填充床电渗泵，并对其性能进行了评价。EOP由三根平行填充的毛细管柱、气体释放装置、铂电极和高压电源组成。对于纯水、纯甲醇、2 mm磷酸二氢钾缓冲液、缓冲液-甲醇混合液、纯水-甲醇混合液和纯水-甲醇混合液，在外加电压小于20W的情况下，EOP可产生大于5.0 Mpa的输出压力和恒定流速。对于甲醇-乙醇-乙腈混合物和甲醇-水混合物，抽气过程中的组成和相对浓度没有明显的变化。对EOP的理论方面进行了详细的讨论。毛细管高效液相色谱系统包括进样阀、进样阀、15厘米×320微米直径、5微米Sphigel C-18不锈钢分析柱和柱上紫外检测器，以评价EOP的性能。在同一系统上，还进行了与机械毛细管高效液相色谱泵的比较研究。结果表明，在毛细管液相色谱应用中，电喷嘴的流速重现性和无脉动流动性能优于机械泵。(C)2004爱思唯尔B.V.保留所有权利。
A packed-bed electroosmotic pump (EOP) was constructed and evaluated. The EOP consisted of three capillary columns packed in parallel, a gas-releasing device, Pt electrodes and a high-voltage power supply. The EOP could generate output pressure above 5.0 MPa and constant flow rate in the range of nl/min to a few mul/min for pure water, pure methanol, 2 mM potassium dihydrogenphosphate buffer, the buffer-methanol mixture and the pure water-methanol mixture at applied potentials less than 20 W The composition of solvent before/after pumping was quantitatively determined by using a gas chromatograph equipped with both flame ionization detector and thermal conductivity detector. It was found that there were no apparent changes in composition and relative concentrations after pumping process for a methanol-ethanol-acetonitrile mixture and a methanol-water mixture. Theoretical aspect of the EOP was discussed in detail. An capillary HPLC system consisting of the EOP, an injection valve, a 15 cm x 320 mum i.d., 5 mum Spherigel C-18 stainless steel analytical column, and an on-column UV detector was connected to evaluate the performance of the EOP. A comparative study was also carried out with a mechanical capillary HPLC pump on the same system. The results demonstrated that the reproducibility of flow rate and the pulsation-free flow property of the EOP are superior to that of mechanical pump in capillary HPLC application. (C) 2004 Elsevier B.V. All rights reserved.