CKLF-Like MARVEL Transmembrane Domain-Containing Member 3 (CMTM3) Inhibits the Proliferation and Tumorigenisis in Hepatocellular Carcinoma Cells.
CKLF-Like MARVEL Transmembrane Domain-Containing Member 3 (CMTM3) Inhibits the Proliferation and Tumorigenisis in Hepatocellular Carcinoma Cells.
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DOI：
10.3727/096504016x14732523471442
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发表时间：
2017-01-26
影响因子：
3.1
通讯作者：
Zhang S
中科院分区：
文献类型：
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作者：
Li W;Zhang S
CKLF样的Marvel跨膜区3(CMTM3)是CMTM家族的成员之一，在多种人类肿瘤中被发现，并在肿瘤的发生发展中起重要作用。然而，CMTM3在肝细胞癌中的作用在很大程度上仍不清楚。因此，在本研究中，我们探讨了它在人肝癌细胞系中的表达模式，以及它在肝癌细胞中的功能。我们的结果表明CMTM3在肝癌细胞系中的表达水平较低。在体外，我们发现CMTM3的过表达明显抑制了肝癌细胞的增殖、侵袭和EMT过程。此外，CMTM3过表达显著下调了HepG2细胞中JAK2和STAT3的磷酸化水平。体内过表达CMTM3可抑制Balb/c裸鼠移植瘤的生长。综上所述，我们证明CMTM3可能通过至少部分抑制JAK2/STAT3信号通路在EMT诱导的肝癌转移中发挥重要作用。因此，CMTM3可能成为预防和/或治疗肝癌侵袭和转移的潜在分子靶点。
The CKLF-like MARVEL transmembrane domain-containing 3 (CMTM3), a member of the CMTM family, was found in several human tumors and plays an important role in the development and progression of tumors. However, the role of CMTM3 in hepatocellular carcinoma (HCC) remains largely unknown. Thus, in the present study, we explored its expression pattern in human HCC cell lines, as well as its functions in HCC cells. Our results demonstrated that the expression of CMTM3 is lowly expressed in HCC cell lines. In vitro, we found that overexpression of CMTM3 obviously inhibited the proliferation, invasion, and EMT process in HCC cells. Furthermore, overexpression of CMTM3 significantly downregulated the expression levels of phosphorylation of JAK2 and STAT3 in HepG2 cells. In vivo, overexpression of CMTM3 attenuated the tumor growth in Balb/c nude mice. In conclusion, we demonstrated that CMTM3 could play an important role in HCC metastasis by EMT induction via, at least partially, suppressing the JAK2/STAT3 signaling pathway. Therefore, CMTM3 may serve as a potential molecular target in the prevention and/or treatment of HCC invasion and metastasis.