Protective Effect of a Rho-kinase Inhibitor on Bladder Dysfunction in a Rat Model of Chronic Bladder Ischemia
Protective Effect of a Rho-kinase Inhibitor on Bladder Dysfunction in a Rat Model of Chronic Bladder Ischemia
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DOI：
10.1016/j.urology.2017.10.007
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发表时间：
2018-01-01
期刊：
影响因子：
2.1
通讯作者：
Kojima, Yoshiyuki
中科院分区：
文献类型：
--
作者：
Akaihata, Hidenori;Nomiya, Masanori;Kojima, Yoshiyuki
目的探讨Rho激酶抑制剂法舒地尔（fasudil）对慢性缺血相关膀胱功能障碍的影响。材料与方法将16周龄雄性SD大鼠分为对照组、慢性膀胱缺血（CBI）组和法舒地尔治疗CBI组（CBI-Fa）。CBI和CBI-Fa组进行双侧髂动脉的球囊内皮损伤，并在手术后接受2%胆固醇饮食8周以诱导CBI。CBI-Fa组在手术后使用zonde口服法舒地尔（30 mg/kg/d）8周。对照组给予常规饮食8周。在清醒状态下的膀胱测压后，各组大鼠被安乐死，并收获的膀胱和髂总动脉的药理学和组织学examination.Results髂总动脉的平均壁厚度是显着大于CBI组比对照组。CBI组大鼠肌条收缩反应明显低于对照组。与对照组相比，CBI组排尿间隔明显缩短，膀胱容量明显降低。在CBI-Fa组中，与CBI组相比，动脉壁增厚被显著抑制。CBI-Fa组与CBI group. CONCLUSION相比，肌条收缩力和膀胱测压参数有显着改善，我们的研究结果表明，法舒地尔慢性治疗可以防止动脉和膀胱功能障碍在这个大鼠模型中的新生内膜形成。法舒地尔在治疗上可用于保护慢性缺血性膀胱的膀胱功能。(c)2017爱思唯尔公司
OBJECTIVE To investigate the effect of fasudil, a Rho-kinase inhibitor, on chronic ischemia-related bladder dysfunction.MATERIALS AND METHODS Male Sprague-Dawley rats (16 weeks old) were divided into control, chronic bladder ischemia (CBI), and CBI with fasudil treatment (CBI-Fa) groups. The CBI and CBI-Fa groups underwent balloon endothelial injury of bilateral iliac arteries and received a 2% cholesterol diet for 8 weeks after the procedure to induce CBI. The CBI-Fa group was given oral fasudil (30 mg/kg/day) using zonde for 8 weeks after the procedure. The control group received a regular diet for 8 weeks. After cystometry in a conscious state, rats from each group were euthanized, and the bladders and common iliac arteries were harvested for pharmacologic and histologic examination.RESULTS Mean wall thickness of the common iliac arteries was significantly greater in the CBI group than in controls. Contractile responses of muscle strips were significantly lower in CBI group rats than in controls. In the CBI group, micturition interval was significantly shorter, and bladder capacity was significantly lower compared with those in controls. In the CBI-Fa group, arterial wall thickening was significantly suppressed compared with the CBI group. Significant improvements in muscle strip contractility and cystometric parameters were seen in the CBI-Fa group compared with the CBI group.CONCLUSION Our results suggest that chronic treatment with fasudil could prevent neointimal formation in arteries and bladder dysfunction in this rat model. Fasudil may be therapeutically useful in protecting bladder function in chronically ischemic bladders. (c) 2017 Elsevier Inc.