Specific oxidative cleavage of carotenoids by VP14 of maize
Specific oxidative cleavage of carotenoids by VP14 of maize
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DOI：
10.1126/science.276.5320.1872
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发表时间：
1997-06-20
期刊：
影响因子：
56.9
通讯作者：
McCarty, DR
中科院分区：
文献类型：
--
作者：
Schwartz, SH;Tan, BC;McCarty, DR
植物生长调节剂脱落酸（ABA）是由环氧类胡萝卜素的氧化裂解形成的。其他类伪胡萝卜素的合成，如动物体内的维生素A，也可能通过类似的机制发生。在ABA生物合成过程中，氧化裂解是第一步反应，被认为是关键的调控步骤。鉴定出一种新的玉米aba缺失突变体，并克隆出相应的基因Vp14。重组VP14蛋白催化9-顺式环氧类胡萝卜素的裂解，形成C-25载醛和叶黄素，叶黄素是高等植物中ABA的前体。
The plant growth regulator abscisic acid (ABA) is formed by the oxidative cleavage of an epoxy-carotenoid. The synthesis of other apocarotenoids, such as vitamin A in animals, may occur by a similar mechanism. In ABA biosynthesis, oxidative cleavage is the first committed reaction and is believed to be the key regulatory step. A new ABA-deficient mutant of maize has been identified and the corresponding gene, Vp14, has been cloned. The recombinant VP14 protein catalyzes the cleavage of 9-cis-epoxy-carotenoids to form C-25 apo-aldehydes and xanthoxin, a precursor of ABA in higher plants.