Functional expression of chemokine receptor CCR6 on human effector memory CD8+ T cells
Functional expression of chemokine receptor CCR6 on human effector memory CD8+ T cells
复制标题
DOI：
10.1002/eji.200636251
复制
发表时间：
2007-01-01
影响因子：
5.4
通讯作者：
Takiguchi, Masafumi
中科院分区：
文献类型：
--
作者：
Kondo, Takaaki;Takata, Hiroshi;Takiguchi, Masafumi
由于CCR 6是在肠和结肠等组织中产生的趋化因子CCL 20的受体，因此认为表达CCR 6的T细胞参与粘膜免疫。CCR 6在人CD 8（+）T细胞上的表达和功能尚未得到很好的分析，尽管已知该受体在人CD 8（+）T细胞的亚群上表达。我们在此描述了人CCR 6（+）CD 8（+）T细胞的特征。多色流式细胞术分析表明，CCR 6（+）细胞主要存在于具有记忆表型的CD 8（+）T细胞中。CCR 6与CCR 5、CCR 7的表达分别呈正相关和负相关。CCR 6 + CD 8（+）T细胞表达颗粒酶A和低水平的穿孔素，但不表达颗粒酶B。此外，这些细胞中的主要群体具有产生IFN-γ和TNF-α但不产生IL-2的能力。这些结果表明，CCR 6（+）CD 8（+）T细胞具有早期效应记忆细胞的特征，而不是效应或中央记忆细胞。趋化性试验显示，CCR 6（+）CD 8（+）T细胞具有响应CCL 20而迁移的能力，表明这些T细胞迁移到组织如结肠并参与粘膜免疫。
Since CCR6 is a receptor for the chemokine CCL20, which is produced in tissues such as intestine and colon, it is thought that T cells expressing CCR6 are involved in mucosal immunity. The expression and function of CCR6 on human CD8(+) T cells have not well been analyzed, although it is known that this receptor is expressed on a subset of human CD8(+) T cells. We here characterize human CCR6(+) CD8(+) T cells. Multi-color flow cytometric analysis demonstrated that CCR6(+) cells are predominantly found among CD8(+) T cells having the memory phenotype. The expression of CCR6 is positively and negatively correlated with that of CCR5 and CCR7, respectively. CCR6+ CD8(+) T cells express granzyme A and a low level of perforin but not granzyme B. In addition, a major population among these cells has the ability to produce IFN-gamma and TNF-alpha but not IL-2. These results indicate that CCR6(+) CD8(+) T cells have characteristics of early effector memory cells rather than effector or central memory cells. A chemotaxis assay revealed that CCR6(+) CD8(+) T cells have the ability to migrate in response to CCL20, suggesting that these T cells migrate to tissues such as colon and are involved in mucosal immunity.