Multiple isotope effects as a probe of proton and hydride transfer in the 6-phosphogluconate dehydrogenase reaction.
Multiple isotope effects as a probe of proton and hydride transfer in the 6-phosphogluconate dehydrogenase reaction.
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多种同位素效应作为 6-磷酸葡萄糖酸脱氢酶反应中质子和氢化物转移的探针。
DOI：
10.1021/bi981288w
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发表时间：
1998
期刊：
影响因子：
--
通讯作者：
Cook,PF
中科院分区：
文献类型：
--
作者：
Hwang,CC;Cook,PF
测定了念珠菌和绵羊肝脏6-磷酸葡萄糖酸脱氢酶(6PGDH)的主溶剂氢、主底物氢、多溶剂氢/底物氢和多溶剂氢/13C同位素对V/K6PG的影响。质子库存数据表明，在氢化物转移之前的一个步骤中存在显著的介质效应，以及在氢化物转移过程中存在动力学溶剂氚同位素效应。多同位素效应数据证实存在多个溶剂氢敏感步骤，可能包括氢化物转移、氢化物转移和脱羧基之前的构象变化。
Primary solvent deuterium, primary substrate deuterium, multiple solvent deuterium/substrate deuterium, and multiple solvent deuterium/13C isotope effects onV/K6PGhave been measured for theCandida utilisand sheep liver 6-phosphogluconate dehydrogenases (6PGDH). Proton inventory data suggest the presence of a significant medium effect in a step preceding hydride transfer and the presence of a kinetic solvent deuterium isotope effect on hydride transfer. Multiple isotope effect data confirm the presence of multiple solvent deuterium sensitive steps, likely including a conformational change preceding hydride transfer, hydride transfer, and decarboxylation.