Cryo-EM is uncovering the mechanism of eukaryotic protein N-glycosylation
Cryo-EM is uncovering the mechanism of eukaryotic protein N-glycosylation
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DOI：
10.1111/febs.14705
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发表时间：
2019-05-01
期刊：
影响因子：
5.4
通讯作者：
Li, Huilin
中科院分区：
文献类型：
--
作者：
Bai, Lin;Li, Huilin
N-glycosylation is one of the predominant modifications of eukaryotic proteins. It is catalyzed by oligosaccharyl transferase (OST), an eight-subunit protein complex in the endoplasmic reticulum membrane. OST transfers the oligosaccharide from a lipid-linked donor (LLO) to the Asn-Xaa-Ser/Thr sequon of nascent polypeptide, usually cotranslationally by partnering with the ribosome and the translocon. We and two other groups have recently determined high-resolution cryo-EM structures of the yeast and mammalian OST complexes. In this Structural Snapshot, we describe the molecular mechanism of eukaryotic OST and its interaction with the translocon.
