Characterization of dicaffeoylspermidine derivatives related glucosyltransferases during fruit development of goji berry.
Characterization of dicaffeoylspermidine derivatives related glucosyltransferases during fruit development of goji berry.
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DOI：
10.1016/j.foodchem.2024.138432
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发表时间：
2024-01
期刊：
影响因子：
8.8
通讯作者：
Xiaoman Yang;Meizhen Yang;Peng Ye;Hanxiang Li;Zhongxi Li;S. Zeng;Ying Wang
中科院分区：
文献类型：
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作者：
Xiaoman Yang;Meizhen Yang;Peng Ye;Hanxiang Li;Zhongxi Li;S. Zeng;Ying Wang
枸杞子（Lycium barbarum，Lb）是一种富含生物活性物质的“超级水果”。在这些化合物中，二咖啡酰亚精胺衍生物（DCSPD）具有抗氧化和抗阿尔茨海默病活性。本研究采用超高效液相色谱-串联质谱法，探讨红枸杞果实发育和成熟过程中的代谢变化。初步鉴定出97种化合物，其中包括51种DCSPD。糖基转移酶（glucosyltransferases，GT）在糖基化DCSPD的形成中起重要作用，体外实验表明3个新的GT都能在N1-咖啡酰-N10-二氢咖啡酰亚精胺上添加一个葡萄糖基。来自mL的Homobox GT。尽管Lr中缺乏丰富的糖基化DCSPD，但Lr表现出类似的活性。这些发现为了解红枸杞中活性化合物的代谢变化和相互关系提供了见解。所鉴定的GT具有DCSPD代谢工程和功能性食品开发的潜力。
The fruit ofLycium barbarum(Lb), known as red goji berry, is a “superfruit” due to its abundance of bioactive compounds. Among these compounds, dicaffeoylspermidine derivatives (DCSPDs) have anti-oxidant and anti-Alzheimer’s Disease activity. This study employed ultra-high-performance liquid chromatography with tandem mass spectrometry to investigate metabolic changes during the development and ripening stages of red goji berries. Totally 97 compounds, including 51 DCSPDs, were tentatively identified. Correlation analysis of these DCSPDs revealed that glycosyltransferases (GTs) play an important role in the formation of glycosylated DCSPDs.In vitroexperiments characterized 3 novel GTs could add a glucosyl moiety toN1-caffeoyl-N10-dihydrocaffeoyl spermidine. Homologous GTs fromL. ruthenicum(Lr) exhibited similar activity, despite the absence of abundant glycosylated DCSPDs in Lr. These findings provide insights into the metabolic changes and interconnections among active compounds in red goji berries. The identified GTs hold potential for metabolic engineering of DCSPDs and functional food development.