Comparative study on adjuvanticity of compound Chinese herbal medicinal ingredients
Comparative study on adjuvanticity of compound Chinese herbal medicinal ingredients
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DOI：
10.1016/j.vaccine.2005.02.011
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发表时间：
2005-05-25
期刊：
影响因子：
5.5
通讯作者：
Liu, JG
中科院分区：
文献类型：
--
作者：
Wang, DY;Hu, YL;Liu, JG
为比较复方中草药成分的佐剂活性，筛选出最佳配方和剂量，制备两种初筛复方中草药成分，分别以3个剂量与纽卡斯尔疫苗混合免疫17日龄雏鸡，以无佐剂和油佐剂疫苗为对照，于免疫后第7、14、21、28、35和42天，预防针用MTT法和血凝抑制试验测定了雏鸡外周血淋巴细胞增殖和血清抗体效价的动态变化。结果表明，两种cCHMI均能促进外周淋巴细胞增殖，增强抗体滴度，其中在某些时间点促进淋巴细胞增殖的佐剂活性与油佐剂相似，甚至强于油佐剂，且存在一定的量效关系。cCHMIs 2在促进细胞免疫方面的最佳佐剂活性出现在中等剂量，而cCHMIs I在增强体液免疫方面的最佳佐剂活性出现在高剂量。两种复方中药注射剂具有良好的协同作用，有望成为替代油佐剂的新型免疫佐剂。(c)2005爱思唯尔有限公司保留所有权利。
In order to compare the adjuvant activity of compound Chinese herbal medicinal ingredients (cCHMIs) and filtrate best prescription and dose, two initial screened cCHMIs were prepared and mixed with Newcastle disease (ND) vaccine respectively at three doses to vaccinate 17-day-old chickens, taking the non-adjuvant and oil adjuvant vaccines as controls, On day 7, 14, 21, 28, 35 and 42 after, vaccination. the dynamic changes of peripheral lymphocyte proliferation and serum antibody titers in the chickens were determined by means of MTT method and hemagglutination inhibition test. The results showed that two cCHMIs could promote peripheral lymphocyte proliferation and enhance antibody titer, of which adjuvant activity was similar to and even stronger than oil adjuvant in promoting lymphocyte proliferation at some time points, and there was a certain dose-effect relationship. The best adjuvant activity of cCHMIs 2 in promoting cellular immunity occurred at middle dose, and cCHMIs I in enhancing humoral immunity, at high dose. Based on good synergistic effects or their components, two cCHMIs would be expected as new-type immunologic adjuvants to substitute for oil adjuvant. (c) 2005 Elsevier Ltd. All rights reserved.