Structures, regulatory regions, and inductive expression patterns of antimicrobial peptide genes in the silkworm Bombyx mori
Structures, regulatory regions, and inductive expression patterns of antimicrobial peptide genes in the silkworm Bombyx mori
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DOI：
10.1016/j.ygeno.2005.11.018
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发表时间：
2006-03-01
期刊：
影响因子：
4.4
通讯作者：
Xiang, ZH
中科院分区：
文献类型：
--
作者：
Cheng, TC;Zhao, P;Xiang, ZH
抗菌肽是一组保护宿主免受感染的免疫蛋白。在果蝇中，已鉴定出七组可诱导的AMP，它们具有抗真菌和革兰氏阳性和革兰氏阴性细菌的活性。根据家蚕基因组序列和表达序列标签，我们鉴定了35个AMP基因，主要属于天蚕素、桑蚕素和Gloverin基因家族。我们预测了基因转录所需的核心启动子以及核因子-kappaB/Rel和GATA转录因子的顺式调控元件。用逆转录聚合酶链式反应检测这些基因在脂多糖免疫攻击后的表达谱。诱导后，抗菌肽B和球蛋白A亚家族成员在脂肪体中有较强的表达。相反，在相同的条件下，Moricin B亚家族的基因不表达。这些结果表明，这些调控元件及其在上游区域的位置在调控这些防御基因的转录方面起着重要作用。(C)2005 Elsevier Inc.保留所有权利。
Antimicrobial peptides (AMPs) are a group of immune proteins that protect the host from infection. In Drosophila, seven groups of inducible AMPs have been identified, with activities against fungi and gram-positive and gram-negative bacteria. On the basis of the silkworm genome sequence and expressed sequence tags, we identified 35 AMP genes, mostly belonging to the cecropin, moricin, and gloverin gene families. We predicted the core promoters required for gene transcription and the cis-regulatory elements for NF-kappa B/Rel and GATA transcription factors. The expression profiles of these genes after an immune challenge with lipopolysaccharide were examined by reverse transcription PCR. Members of the cecropin B and gloverin A subfamilies were intensely expressed in the fat body after induction. In contrast, those of the moricin B subfamily were not expressed under the same conditions. Such results suggest that these regulatory elements and their positions in the upstream regions play an important role in regulating the transcription of these defense genes. (c) 2005 Elsevier Inc. All rights reserved.