pDNAVACCultra vector family: high throughput intracellular targeting DNA vaccine plasmids
pDNAVACCultra vector family: high throughput intracellular targeting DNA vaccine plasmids
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DOI：
10.1016/j.vaccine.2005.08.033
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发表时间：
2006-05-22
期刊：
影响因子：
5.5
通讯作者：
Hodgson, Clague
中科院分区：
文献类型：
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作者：
Williams, Jarnes A.;Luke, Jeremy;Hodgson, Clague
DNA 疫苗有可能为针对肿瘤和传染原的保护性免疫提供安全途径。然而，当前的 DNA 疫苗质粒对于额外的非必需 DNA 并不是最佳的，也不能促进抗原受控或灵活地靶向各种细胞内目的地。构建了一系列 DNA 疫苗载体，并对其进行了优化和最小化，以符合 FDA 关于含量和消除外来物质的指南。所得载体比现有载体小得多，驱动更高水平的靶基因表达，促进高通量克隆应用，并允许同时克隆到具有蛋白质产物的各种细胞内靶向目的地的多个载体中。控制表达和运输的能力旨在为癌症治疗和新出现的传染病提供快速、合理的方法。 (c) 2005 Elsevier Ltd. 保留所有权利。
DNA vaccines have the potential to provide a safe route for protective immunity to neoplasms and infectious agents. However, current DNA vaccine plasmids are not optimal with additional non-essential DNA, nor do they facilitate controlled or flexible targeting of antigens to various intracellular destinations. A family of DNA vaccine vectors, optimized and minimized to comply with FDA guidelines regarding content and elimination of extraneous materials, was constructed. The resulting vectors are much smaller than existing vectors, drive higher levels of target gene expression, facilitate high throughput cloning applications, and allow simultaneous cloning into multiple vectors that feature various intracellular targeting destinations for the protein product. The ability to control expression and trafficking is intended to provide a rapid, rational approach to cancer therapy and emerging infectious diseases. (c) 2005 Elsevier Ltd. All rights reserved.