Measurement of adhesive forces between S-epidermidis and fibronectin-coated surfaces using optical tweezers
Measurement of adhesive forces between S-epidermidis and fibronectin-coated surfaces using optical tweezers
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DOI：
10.1002/lsm.10070
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发表时间：
2002-01-01
影响因子：
2.4
通讯作者：
Anvari, B
中科院分区：
文献类型：
--
作者：
Simpson, KH;Bowden, MG;Anvari, B
背景和目标：生物材料介导的感染是医疗器械失效的常见原因，由细菌粘附到植入物表面的吸附蛋白层引发。这种粘附被认为是由细菌细胞表面上存在的特定分子介导的。光镊可用于测量单个细菌和蛋白质包被的surfaces.Study设计/材料和方法之间的粘附力：使用光镊，细菌被捕获，并与10 μ m直径的聚苯乙烯微球与纤连蛋白包被接触。在一定的纤连蛋白浓度和接触时间范围内测定了细胞从微珠上分离所需的最小力。结果：分离力是18 pN基础值的整数倍，与接触时间和涂层浓度无关;我们提出力的变化与形成的键数有关。这些实验表明，光镊可用于研究单个细菌对表面的粘附。结果表明，S.表皮具有能够结合纤连蛋白的表面蛋白。(C)2002 Wiley-Liss，Inc.
Background and Objectives: Biomaterial-mediated infection, a common cause of medical device failure, is initiated by bacterial adhesion to an adsorbed protein layer on the implant surface. This adhesion is thought to be mediated by specific molecules present on the bacterial cell surface. Optical tweezers can be used to measure the adhesive force between a single bacterium and a protein-coated surface.Study Design/Materials and Methods: Using optical tweezers, a bacterium was trapped and brought in contact with a 10-mum diameter polystyrene microsphere coated with fibronectin. The minimum force required to detach the cell from the bead was determined over a range of fibronectin concentrations and contact times.Results: The detachment forces were integer multiples of an 18-pN base value that was independent of contact time and coating concentration; we propose that the variation in force is related to the number of bonds formed.Conclusions: These experiments demonstrate that optical tweezers can be used to investigate the adhesion of individual bacteria to surfaces. The results suggest that S. epidermidis has surface proteins capable of binding fibronectin. (C) 2002 Wiley-Liss, Inc.