Species diversity of yeast in oral colonization of insulin-treated diabetes mellitus patients
Species diversity of yeast in oral colonization of insulin-treated diabetes mellitus patients
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DOI：
10.1007/s11046-006-0038-5
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发表时间：
2006-08-01
期刊：
影响因子：
5.5
通讯作者：
Soares Mendes Giannini, Maria Jose
中科院分区：
文献类型：
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作者：
Helena Pires Goncalves, Regina;Miranda, Elaine Toscano;Soares Mendes Giannini, Maria Jose
本研究的目的是调查胰岛素依赖型糖尿病患者（175）的口腔酵母菌定植、抗真菌药敏性和菌株多样性，并评估牙科修复体的影响。将口腔冲洗液样品在选择性培养基上培养，以分离、计数和鉴定回收的酵母菌。超过一半的糖尿病受试者（53%）在口腔中携带大量的念珠菌细胞，这些生物体在戴假牙的糖尿病患者中以更高的密度回收。从这些患者中共分离出93株酵母菌，包括：念珠菌属。（n = 89）;毕赤酵母属（n = 02）;丝孢酵母属（n = 1）和地霉属（n = 1）。C.其中白色念珠菌占56%，非白色念珠菌占39.8%，其它属占4.3%。C.白色念珠菌占优势，其次为念珠菌;近平滑C. tropicalis、热带假丝酵母C.光叶C. krusei，C. rugosa和C.季也蒙地。琼脂扩散法检测非白念珠菌及其它属酵母菌对氟康唑的耐药率为21.9%，以念珠菌为主。rugosa（100%）、C. glabrata（57%）和C. krusei（50%）。与糖尿病相关的局部口腔因素，如假牙的存在，似乎具有增加口腔中念珠菌属的数量和种类的作用，主要是那些药物敏感性较低的念珠菌。
The aim of this study was to investigate oral yeast colonization, antifungal susceptibility and strain diversity in insulin-dependent diabetes mellitus patients (175), as well as to evaluate the influence of dental prostheses. Oral rinse samples were cultured on selective media, in order to isolate, count and identify the yeasts recovered. More than half of the diabetic subjects (53%) carried significant amounts of Candida cells in the buccal cavity and these organisms were recovered at higher densities in diabetics wearing dentures. A total of 93 yeast strains were isolated from these patients, including: Candida spp. (n = 89); Pichia (n = 02); Trichosporon (n = 1), and Geotrichum (n = 1). C. albicans represented 56% of these strains, non-albicans Candida 39.8%, and other genera of yeast 4.3%. C. albicans was prevalent, followed by C. parapsilosis, C. tropicalis, C. glabrata, C. krusei, C. rugosa and C. guilliermondii. Agar disk-diffusion tests of the susceptibility of non-albicans Candida and other genera of yeast to fluconazole showed resistance in 21.9%, mainly in C. rugosa (100%), C. glabrata (57%) and C. krusei (50%). Local oral factors, such as the presence of dentures, in association with diabetes, seemed to have the effect of increasing the amount and variety of Candida species in the oral cavities, mainly those with lower drug susceptibilities.