INTESTINAL AND LIVER FATTY-ACID-BINDING PROTEINS DIFFERENTIALLY AFFECT FATTY-ACID UPTAKE AND ESTERIFICATION IN L-CELLS
INTESTINAL AND LIVER FATTY-ACID-BINDING PROTEINS DIFFERENTIALLY AFFECT FATTY-ACID UPTAKE AND ESTERIFICATION IN L-CELLS
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DOI：
10.1007/bf02537481
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发表时间：
1995-10-01
期刊：
影响因子：
1.9
通讯作者：
SCHROEDER, F
中科院分区：
文献类型：
--
作者：
PROWS, DR;MURPHY, EJ;SCHROEDER, F
在转基因小鼠L细胞成纤维细胞上，观察了肠脂肪酸结合蛋白和肝脏脂肪酸结合蛋白对脂肪酸摄取和酯化的不同影响。与对照细胞相比，L-FABP的表达，而不是I-FABP的表达，使顺式巴豆酸摄取的初始速率和程度分别增加了50%和29%。I-FABP和L-FABP的表达使[H-3]-油酸掺入三酰甘油的比例分别增加5.5倍和3.8倍。而L和i-FABP均能促进[H-3]-油酸酯化生成乙醇胺甘油磷脂，但对酯化生成胆碱甘油磷脂的作用相反。这些数据首次表明，不同的FABP对脂肪酸的摄取和细胞内的酯化都有不同的影响。
Differential effects of intestinal (I-FABP) or liver (L-FABP) fatty acid binding proteins on fatty acid uptake and esterification were examined using transfected mouse L-cell fibroblasts. L-FABP, but not I-FABP, expression increased the initial rate and extent of cis-parinaric acid uptake by 50 and 29%, respectively, compared to control cells. I-FABP and L-FABP expression preferentially increased [H-3]-oleic acid incorporation into triacylglycerols by 5.5-fold and 3.8-fold, respectively. While both L-FABP and I-FABP increased esterification of [H-3]-oleic acid into ethanolamine glycerophospholipids, these proteins had opposite effect on esterification into choline glycerophospholipids. These data show for the first time that distinct FABP differentially affect both fatty acid uptake and intracellular esterification.