TRANSGANGLIONIC LABELING OF PRIMARY SENSORY AFFERENTS IN THE RAT LUMBAR SPINAL-CORD - COMPARISON BETWEEN WHEAT-GERM-AGGLUTININ AND THE I-B4 ISOLECTIN FROM BANDEIRAEA-SIMPLICIFOLIA
TRANSGANGLIONIC LABELING OF PRIMARY SENSORY AFFERENTS IN THE RAT LUMBAR SPINAL-CORD - COMPARISON BETWEEN WHEAT-GERM-AGGLUTININ AND THE I-B4 ISOLECTIN FROM BANDEIRAEA-SIMPLICIFOLIA
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DOI：
10.1007/bf01268087
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发表时间：
1994-12-01
期刊：
影响因子：
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通讯作者：
SNOW, PJ
中科院分区：
文献类型：
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作者：
KITCHENER, PD;LAPIZ, MDS;SNOW, PJ
我们最近报道了单叶板栗I-B-4异凝素可以作为一种跨神经节的神经元示踪剂，它似乎对背部浅部的无髓皮肤传入(C纤维)及其终末具有选择性。由于浅背角的终末也被麦芽凝集素标记，我们试图比较这两种神经元示踪剂。成年大鼠坐骨神经注射1%麦芽凝集素-HRP或1%BSI-B-4-HRP 3天后，取腰髓行HRP反应。标记结构多见于背角浅层，上覆白质(包括Lissauer束)的标记结构较少。在麦芽凝集素-HRP实验中，大多数标记的结构是突触终末(63%)和无髓轴突(32%)。麦芽凝集素-HRP标记结构中，约3%为细小的有髓纤维(仅见于板层I和外板II)，约2%位于神经元体。相反，BSI-B-4-HRP实验仅在突触终末(37%)和无髓轴突(63%)中发现标记。以往的研究表明，小直径的背根节神经元及其在浅背角的终末表达一系列结构上相关的碳水化合物，这些碳水化合物含有BSI-B-4和/或小麦胚凝集素的结合部位。本研究中两种跨神经节示踪剂所产生的标记模式的比较表明，无髓鞘坐骨神经传入表达麦芽凝集素和BSI-BB结合位点，但一些细小的有髓传入和位于I/II外板的一种不同形式的突触终末表达的是麦芽凝集素结合位点，而不是BSI-B-4结合位点。
We recently reported that the I-B-4 isolectin from Bandeiraea simplicifolia could be used as a transganglionic neuronal tracer which appears to be selective for unmyelinated cutaneous afferents (C fibres) and their terminals in the superficial dorsal hem. As terminals in the superficial dorsal horn are also labelled by wheatgerm agglutinin, we sought to compare these two neuronal tracers. Three days after the injection of 1% wheatgerm agglutinin-HRP or 1% BSI-B-4-HRP into the sciatic nerve of adult rats the lumbar spinal cord was processed for HRP reactivity. The majority of labelled structures was found in the superficial dorsal horn, with fewer labelled structures seen in the overlying white matter (including Lissauer's tract). In wheatgerm agglutinin-HRP experiments most labelled structures were synaptic terminals (63%) and unmyelinated axons (32%). About 3% of wheatgerm agglutinin-HRP-labelled structures were fine myelinated fibres (which were found only in lamina I and outer lamina II) and about 2% of label was located in neuronal somata. In contrast, label from BSI-B-4-HRP experiments was found only in synaptic terminals (37%) and unmyelinated axons (63%). Previous studies have shown that small diameter dorsal root ganglion neurons and their terminals in the superficial dorsal horn express a range of structurally related carbohydrates that contain binding sites for BSI-B-4 or wheatgerm agglutinin or both. Comparison of the labelling patterns produced by the two transganglionic tracers in the present study suggests that unmyelinated sciatic afferents express wheatgerm agglutinin and BSI-BB binding sites, but some thin myelinated afferents, and a distinct form of synaptic terminal in lamina I/II outer, express the wheatgerm agglutinin binding site and not the BSI-B-4 binding site.