Dynamic Expression Pattern of SERPINA1 Gene from Duck (Anas platyrhynchos)
Dynamic Expression Pattern of SERPINA1 Gene from Duck (Anas platyrhynchos)
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鸭 (Anas platyrhynchos) SERPINA1 基因的动态表达模式
DOI：
10.1155/2019/1321287
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发表时间：
2019-01-01
影响因子：
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通讯作者：
Chen, Guohong
中科院分区：
文献类型：
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作者：
Gu, Tiantian;Wu, Ningzhao;Chen, Guohong
SERPINA1是丝氨酸蛋白酶抑制剂的一员，越来越多地被认为是人类和动物先天免疫的调节因子。然而，SERPINA1基因在鸭免疫防御病毒感染中的表达和功能尚不清楚。采用逆转录聚合酶链反应（RT-PCR）和cDNA末端快速扩增（RACE）技术获得du SERPINA1 cDNA全长序列。它包含1457个核苷酸，包括47-bp的5' UTR、135-bp的3' UTR和1275-bp的开放阅读框（ORF），编码一个424个氨基酸的蛋白。然后，测定du SERPINA1基因的组织表达谱。实时定量聚合酶链反应（Real-time qPCR）分析显示，du SERPINA1 mRNA在各组织中普遍存在，但在肺和肝组织中表达水平较高。此外，还研究了鸭肝炎病毒1型（DHV-1）和多核糖苷型多核糖苷酸（poly I:C）攻毒雏鸭时的表达谱。DHV-1注射或poly I:C处理后，肝脏和肾脏组织中du SERPINA1 mRNA表达上调。但两种组织的峰值时间并不一致。在肾脏中，SERPINA1的表达水平在治疗后立即升高，而在肝组织中，SERPINA1的表达水平保持稳定，直到感染后12 h。我们的研究结果表明，SERPINA1在抗病毒反应中具有积极作用，从而提高了我们对该蛋白作用的理解。
SERPINA1 is a member of serine protease inhibitors and is increasingly considered to be a regulator of innate immunity in human and animals. However, the expression and function of SERPINA1 gene in immune defense against viral infection remain unknown in ducks. The full-length du SERPINA1 cDNA sequence was obtained using reverse transcription polymerase chain reaction (RT-PCR) and rapid amplification of cDNA ends (RACE). It contained 1457 nucleotide, including 47-bp 5' UTR, 135-bp 3' UTR, and 1275-bp open reading frame (ORF), and encodes a 424-amino acid protein. Then, the tissue expression profile of du SERPINA1 gene was determined. Real-time quantitative polymerase chain reaction (real-time qPCR) analysis revealed that du SERPINA1 mRNA is ubiquitous in various tissues, but higher expression levels were observed in lung and liver tissues. In addition, the expression pattern was investigated when the ducklings were challenged with duck hepatitis virus 1(DHV-1) and polyriboinosinic polyribocytidylic acid (poly I:C). After DHV-1 injection or poly I:C treatment, du SERPINA1 mRNA was up-regulated in the liver and kidney tissues. However, the peak time in two tissues was not consistent. In kidney, the expression lever of SERPINA1 increased immediately after the treatment while in liver tissue it kept steady until 12 h post-infection. Our results indicate that SERPINA1 has an active role in the antiviral response, and thus improve our understanding of the role of this protein.