Fabrication of Hyaluronan-Poly(vinylphosphonic acid)-Chitosan Hydrogel for Wound Healing Application
Fabrication of Hyaluronan-Poly(vinylphosphonic acid)-Chitosan Hydrogel for Wound Healing Application
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DOI：
10.1155/2016/6723716
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发表时间：
2016-01-01
影响因子：
3.3
通讯作者：
Bui Chi Bao
中科院分区：
文献类型：
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作者：
Dang Hoang Phuc;Nguyen Thi Hiep;Bui Chi Bao
制备了由透明质酸、聚（乙烯基膦酸）和壳聚糖（HA/PVPA/CS水凝胶）制成的新型水凝胶，并表征其用于皮肤伤口愈合应用。首先，研究了水凝胶的组分比以优化反应效率。其次，通过光学显微镜观察其微观结构。通过核磁共振光谱和傅立叶变换红外光谱评价了水凝胶中的化学相互作用。然后，对其降解速率进行了研究。然后，通过琼脂扩散法检测水凝胶的抗菌活性。最后，进行体内研究以评价水凝胶的生物相容性。结果表明，优化后的水凝胶具有三维高孔结构，孔径范围在25 μ m ~ 125 μ m之间。此外，其降解时间为两周，可以为重塑阶段的细胞外基质框架形成提供足够的时间。抑菌试验表明，该水凝胶对大肠杆菌具有一定的抑菌活性.杆菌最后，体内研究表明，水凝胶不会被免疫系统排斥，并且可以促进伤口愈合过程。总的来说，HA/PVPA/CS水凝胶被成功地制造，并且结果暗示其用于伤口愈合应用的潜力。
A new hydrogel made of hyaluronan, poly(vinylphosphonic acid), and chitosan (HA/PVPA/CS hydrogel) was fabricated and characterized to be used for skin wound healing application. Firstly, the component ratio of hydrogel was studied to optimize the reaction effectiveness. Next, its microstructure was observed by light microscope. The chemical interaction in hydrogel was evaluated by nuclear magnetic resonance spectroscopy and Fourier transform-infrared spectroscopy. Then, a study on its degradation rate was performed. After that, antibacterial activity of the hydrogel was examined by agar diffusion method. Finally, in vivo study was performed to evaluate hydrogel's biocompatibility. The results showed that the optimized hydrogel had a three-dimensional highly porous structure with the pore size ranging from about 25 mu m to less than 125 mu m. Besides, with a degradation time of two weeks, it could give enough time for the formation of extracellular matrix framework during remodeling stages. Furthermore, the antibacterial test showed that hydrogel has antimicrobial activity against E. coli. Finally, in vivo study indicated that the hydrogel was not rejected by the immune system and could enhance wound healing process. Overall, HA/PVPA/CS hydrogel was successfully fabricated and results implied its potential for wound healing applications.