Spatial and vertical distribution of bacteria in the Pearl River estuary sediment
Spatial and vertical distribution of bacteria in the Pearl River estuary sediment
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DOI：
10.5897/ajb11.568
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发表时间：
2012-01
影响因子：
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通讯作者：
F. Sun;You-Shao Wang;Yu-Tu Wang;Qian P. Li
中科院分区：
文献类型：
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作者：
F. Sun;You-Shao Wang;Yu-Tu Wang;Qian P. Li
为了研究珠江口沉积物中细菌群落结构的空间和垂直变化，采用变性梯度凝胶电泳（DGGE）和多元统计分析方法进行了研究。多维尺度分析（MDS）结果与DGGE带图吻合较好，表明除A2样品外，垂直深度对沉积物细菌群落结构有显著影响。通过典型对应分析（CCA）来推断环境变量与细菌群落结构之间的关系。不同站点的细菌种类与上覆水环境有着独特的密切关系。盐度、pH、铵盐、磷酸盐和硅酸盐被认为是影响细菌群落组成变化的关键因素。邻近结合分析将细菌序列分为8个类群，分别是变形菌门（Proteobacteria）、酸性菌门（Acidobacteria）、拟杆菌门（Bacteroidetes）、绿杆菌门（Chloroflexi）、放线菌门（Actinobacteria）、厚壁菌门（Firmicutes）、plantomycetales和蓝藻门（Cyanobacteria）。测序分析结果表明，变形菌门和拟杆菌门是4个沉积物样品中的优势菌群。
In order to investigate the spatial and vertical change of bacteria community structure in the Pearl River estuary sediment, denaturing gradient gel electrophoresis (DGGE) and multivariate statistical analyses were carried out in this study. Results of multidimensional scaling analyses (MDS) were in good agreement with the DGGE band patterns suggesting that vertical depth had a significant impact on sediment bacterial community structure except sample A2. Canonical correspondence analysis (CCA) was also conducted to infer the relationship between environmental variables and bacterial community structure. Bacterial phylotypes in different stations were closely related uniquely to the overlying water environment. Salinity, pH, ammonium, phosphate and silicate were considered to be the key factors driving the changes in bacterial community composition. The neighbour-joining analysis divided bacteria sequences into eight groups, Proteobacteria, Acidobacteria, Bacteroidetes, Chloroflexi, Actinobacteria, Firmicutes, Planctomycetales and Cyanobacteria. Sequencing analysis results suggested that Proteobacteria and Bacteroidetes were the dominant bacterial groups in the four sediment samples.