Modulating immune responses with probiotic bacteria
Modulating immune responses with probiotic bacteria
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DOI：
10.1046/j.1440-1711.2000.00887.x
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发表时间：
2000-02-01
影响因子：
4
通讯作者：
Chin, J
中科院分区：
文献类型：
--
作者：
Matsuzaki, T;Chin, J
多年来，已知益生菌为消费者带来健康益处。一种可能的机制可能是益生菌调节免疫反应的能力。已发现口服施用干酪乳杆菌菌株Shirota（LcS）通过刺激脾NK细胞的活性来增强先天免疫。口服喂养杀死LcS能够刺激生产的Th1细胞因子，导致抑制生产的IgE抗体对卵清蛋白在实验小鼠。利用益生菌将粘膜免疫应答向Th1转变的能力提供了治疗过敏性疾病的策略。胸膜内注射LcS也可抑制肺中甲氨蝶呤肿瘤细胞的生长。口服其他益生菌，如嗜热链球菌（St），发酵乳杆菌（Lf）和酵母（Y），引起不同的免疫反应。预先喂食酵母或Lf，然后喂食卵清蛋白（OVA）的小鼠对接种OVA的反应比未给予益生菌或OVA的小鼠或仅预先喂食OVA的小鼠更好。然而，在预先喂饲酵母然后喂饲酵母和OVA的小鼠以及预先喂饲Lf然后喂饲Lf和OVA的小鼠中，响应于接种OVA的抗体应答被显著抑制。先喂St后喂OVA增强了对卵清蛋白的细胞免疫应答。相比之下，先喂St再喂St + OVA的小鼠对OVA反应迟钝。虽然单独的抗原喂养似乎引发免疫应答，但抗原与益生菌的共喂养可以抑制抗体和细胞免疫应答，并且可以通过联合给予髓鞘碱性蛋白和益生菌提供有效的方案来减弱自身免疫性疾病，例如实验性过敏性脑脊髓炎。
For many years, probiotic bacteria have been known to confer health benefits to the consumer. One possible mechanism for this may be the ability of probiotic bacteria to modulate immune responses. Oral administration of Lactobacillus casei strain Shirota (LcS) has been found to enhance innate immunity by stimulating the activity of splenic NK cells. Oral feeding with killed LcS was able to stimulate the production of Th1 cytokines, resulting in repressed production of IgE antibodies against Ovalbumin in experimental mice. The ability to switch mucosal immune responses towards Th1 with probiotic bacteria provides a strategy for treatment of allergic disorders. Growth of Meth A tumour cells in the lungs was also inhibited by intrapleural injection of LcS. Oral administration of other probiotic bacteria, such as Streptococcus thermophilus (St), Lactobacillus fermentum (Lf) and yeast (Y), elicited different immune responses. Mice that were prefed yeast or Lf followed by feeding with ovalbumin (OVA) responded better to vaccination with OVA than mice not given either probiotic or OVA or mice that had been prefed only OVA. However, antibody responses were significantly suppressed in response to vaccination with OVA in mice that had been prefed yeast followed by yeast and OVA as well as mice prefed Lf followed by Lf and OVA. Prefeeding St followed by OVA feeding enhanced cellular immune responses against ovalbumin. In contrast, mice prefed St followed by St + OVA were hyporesponsive against OVA. While antigen feeding alone appears to prime for an immune response, cofeeding antigen with probiotic bacteria can suppress both antibody and cellular immune responses and may provide an efficacious protocol to attenuate autoimmune diseases, such as experimental allergic encephalomyelitis, by jointly dosing with myelin basic protein and probiotic bacteria.