The type VII secretion system protects Staphylococcus aureus against antimicrobial host fatty acids
The type VII secretion system protects Staphylococcus aureus against antimicrobial host fatty acids
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VII 型分泌系统保护金黄色葡萄球菌免受抗菌宿主脂肪酸的侵害
DOI：
10.1101/572172
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发表时间：
2019
期刊：
影响因子：
--
通讯作者：
Tchoupa A
中科院分区：
文献类型：
--
作者：
Tchoupa A
金黄色葡萄球菌VII型分泌系统（T7SS）输出几种对细菌毒力至关重要的蛋白质。然而，T7SS介导的葡萄球菌在感染过程中存活的机制仍不清楚。在这里，我们报告说，S。缺乏T7SS组分的金黄色葡萄球菌对宿主来源的抗微生物脂肪酸更敏感。不饱和脂肪酸如亚油酸（LA）引起的抑制增加S。与野生型（WT）相比，金黄色葡萄球菌突变体缺乏T7SS效应子EsxC、EsxA和EsxB，或膜结合的ATP酶EsxC。T7SS突变体在不同的S.金黄色葡萄球菌菌株背景也显示出对LA的敏感性增加。细菌膜脂质分布的分析表明，theesxCmutant是不太能够将LA纳入其膜磷脂。尽管WT和突变株之间结合标记LA的能力没有差异，但与WT相比，LA在T7SS突变株中诱导更多的细胞膜损伤。此外，WT和突变体细胞组分的蛋白质组学分析显示，除了损害膜，T7SS缺陷诱导氧化应激并阻碍它们对LA挑战的反应。因此，我们的研究结果表明，T7SS有助于促进衰老。当细菌遇到抗微生物脂肪酸时，金膜完整性和体内平衡。
TheStaphylococcus aureustype VII secretion system (T7SS) exports several proteins that are pivotal for bacterial virulence. The mechanisms underlying T7SS-mediated staphylococcal survival during infection nevertheless remain unclear. Here we report thatS. aureuslacking T7SS components are more susceptible to host-derived antimicrobial fatty acids. Unsaturated fatty acids such as linoleic acid (LA) elicited an increased inhibition ofS. aureusmutants lacking T7SS effectors EsxC, EsxA and EsxB, or the membrane-bound ATPase EssC, compared to the wild-type (WT). T7SS mutants generated in differentS. aureus strain backgrounds also displayed an increased sensitivity to LA. Analysis of bacterial membrane lipid profiles revealed that theesxCmutant was less able to incorporate LA into its membrane phospholipids. Although the ability to bind labelled LA did not differ between the WT and mutant strains, LA induced more cell membrane damage in the T7SS mutants compared to the WT. Furthermore, proteomic analyses of WT and mutant cell fractions revealed that, in addition to compromising membranes, T7SS defects induce oxidative stress and hamper their response to LA challenge. Thus, our findings indicate that T7SS contribute to maintainingS. aureusmembrane integrity and homeostasis when bacteria encounter antimicrobial fatty acids.
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影响因子：
2.8
作者：
Neumann, Yvonne;Ohlsen, Knut;Donat, Stefanie;Engelmann, Susanne;Kusch, Harald;Albrecht, Dirk;Cartron, Michael;Hurd, Alexander;Foster, Simon J.
通讯作者：
Foster, Simon J.
影响因子：
5.7
作者：
Cruciani M;Etna MP;Camilli R;Giacomini E;Percario ZA;Severa M;Sandini S;Rizzo F;Brandi V;Balsamo G;Polticelli F;Affabris E;Pantosti A;Bagnoli F;Coccia EM
通讯作者：
Coccia EM
EssC：结构域结构揭示了 VII 型分泌系统中难以捉摸的易位通道
影响因子：
4.1
作者：
M. Zoltner;W. Ng;J. J. Money;P. Fyfe;H. Kneuper;T. Palmer;W. Hunter
通讯作者：
W. Hunter
EssC 是金黄色葡萄球菌 VII 型分泌的特异性决定因素
DOI：
10.1101/252353
发表时间：
2018
期刊：
--
影响因子：
--
作者：
Jäger F
通讯作者：
Jäger F
影响因子：
3.6
作者：
Pulschen, Andre A.;Sastre, Diego E.;Gueiros-Filho, Frederico J.
通讯作者：
Gueiros-Filho, Frederico J.