TROP2 increases growth and metastasis of human oral squamous cell carcinoma through activation of the PI3K/Akt signaling pathway
TROP2 increases growth and metastasis of human oral squamous cell carcinoma through activation of the PI3K/Akt signaling pathway
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DOI：
10.3892/ijmm.2019.4378
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发表时间：
2019-12-01
影响因子：
5.4
通讯作者：
Feng, Zhengqing
中科院分区：
文献类型：
--
作者：
Tang, Genxiong;Tang, Qi;Feng, Zhengqing
口腔的大多数恶性肿瘤是口腔鳞状细胞癌（OSCC），这是一种高度恶性的肿瘤，具有形成远处转移的倾向。滋养层细胞表面抗原2（TROP 2）是一种跨膜蛋白，在几种类型的肿瘤细胞中过表达，但其在OSCC中的作用和调节机制尚未确定。本研究的目的是检测TROP 2在人口腔鳞癌细胞系中的作用。本研究表明，TROP 2蛋白表达上调，在口腔鳞癌细胞系。转染靶向TROP 2的短发夹状RNA（sh-TROP 2）可降低OSCC细胞的增殖、迁移和侵袭能力，而过表达TROP 2则可增加OSCC细胞的增殖、迁移和侵袭能力。在OSCC细胞系中转染sh-TROP 2抑制OSCC小鼠模型中的肿瘤生长。此外，TROP 2的表达激活了人口腔鳞癌细胞中的磷酸肌醇3-激酶（PI 3 K）/Akt信号通路。这些结果表明，TROP 2通过激活PI 3 K/Akt信号通路诱导OSCC细胞的生长、迁移和侵袭。
Most malignant neoplasms of the oral cavity are oral squamous cell carcinoma (OSCC), which is a type of highly malignant tumor with a propensity for forming distant metastases. Trophoblast cell surface antigen 2 (TROP2) is a transmembrane protein that is overexpressed in several types of tumor cells, although its role and regulatory mechanism in OSCC have not been determined. The aim of the present study was to examine the effects of TROP2 in human OSCC cell lines. The present study demonstrated that TROP2 protein expression was upregulated in OSCC cell lines. Transfection of short hairpin RNA (shRNA) targeting TROP2 (sh-TROP2) reduced cell proliferation, migration and invasion of OSCC cell lines, whereas overexpression of TROP2 increased proliferation, migration and invasion. sh-TROP2 transfection in OSCC cell lines inhibited tumor growth in OSCC mouse models. Furthermore, TROP2 expression activated the phosphoinositide 3-kinase (PI3K)/Akt signaling pathway in human OSCC cells. These results suggest that TROP2 induces cell growth, migration and invasion through activation of the PI3K/Akt signaling pathway in OSCC cells.