A human IAP-family gene, Apollon, expressed in human brain cancer cells
A human IAP-family gene, Apollon, expressed in human brain cancer cells
复制标题
DOI：
10.1006/bbrc.1999.1585
复制
发表时间：
1999-11-02
影响因子：
3.1
通讯作者：
Tsuruo, T
中科院分区：
文献类型：
--
作者：
Chen, ZH;Naito, M;Tsuruo, T
IAP是具有杆状病毒IAP重复序列（BIR）结构域并抑制细胞凋亡的蛋白质家族。我们发现了一个人类IAP家族基因，我们命名为Apollon，编码一个巨大的蛋白质（530 kDa），包含一个单一的BIR结构域和一个泛素结合酶结构域，这是一个人类同源物的布鲁斯。在我们检测的38个人类癌细胞系中，Apollon蛋白在六种脑癌（神经胶质瘤）中的四种和五种卵巢癌中的一种中表达。在脑癌细胞系中，SNB-78高水平表达Apollon，并且该细胞系显示出对各种抗癌药物的抗性。Apollon反义寡核苷酸处理SNB-78细胞后，Apollon蛋白表达降低，对顺铂和喜树碱诱导的细胞凋亡敏感。这些结果表明，Apollon保护SNB-78细胞免于凋亡，并且至少部分地在该细胞系的肿瘤发生和耐药性中起作用。(C)北京：科学出版社.
IAP is a family of protein that has baculovirus IAP repeat (BIR) domains and inhibits apoptosis. We found a human IAP family gene, which we named Apollon, encoding a huge protein (530 kDa) that contains a single BIR domain and a ubiquitin-conjugating enzyme domain, that is a human homolog of BRUCE. Apollon protein was expressed in four of six brain cancers (gliomas), and one of five ovarian cancers in 38 human cancer cell lines that we examined. Among the brain cancer cell lines, SNB-78 expressed a high level of Apollon, and this cell line shows resistance against various anticancer drugs. Treating SNB-78 cells with antisense oligonucleotide against Apollon reduced the expression of Apollon protein, and significantly sensitized the cells to apoptosis induced by cisplatin and camptothecin. These results suggest that Apollon protects SNB-78 cells from undergoing apoptosis and, at least in part, plays a role in tumorigenesis and drug resistance of this cell line. (C) 1999 Academic Press.