The T-lineage-affiliated CD2 gene lies within an open chromatin environment in acute promyelocytic leukemia cells.
The T-lineage-affiliated CD2 gene lies within an open chromatin environment in acute promyelocytic leukemia cells.
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T 谱系相关的 CD2 基因位于急性早幼粒细胞白血病细胞的开放染色质环境中。
作者：
D. Grimwade;S. Outram;R. Flora;S. Ings;A. Pizzey;R. Morilla;C. Craddock;D. Linch;E. Solomon
急性髓性白血病（AML）中发生白血病转化的造血祖细胞的性质尚未明确定义。为了解决这个问题，我们使用DNase I超敏试验来研究急性早幼粒细胞型AML （APL）中t系相关CD2基因周围的染色质结构。在原代APL细胞中发现CD2的上游和下游侧边区域对DNase I敏感，与t系细胞中检测到的敏感位点模式相同。在b系白血病细胞中，所有这些位点都被证实是DNase I无法到达的。原发性APL细胞中t细胞相关染色质特征的证明，对APL起源的影响可能比以前认为的更原始的祖细胞。
The nature of hemopoietic progenitors subject to leukemic transformation in acute myeloid leukemia (AML) has not been clearly defined. To address this issue, we have used DNase I hypersensitivity assays to study the chromatin structure surrounding the T-lineage-affiliated CD2 gene in the acute promyelocytic subtype of AML (APL). Upstream and downstream flanking regions of CD2 were found to be hypersensitive to DNase I in primary APL blasts, with an identical pattern of hypersensitive sites to those detected in cells of T-lineage. All of the sites were confirmed to be inaccessible to DNase I in B-lineage leukemia cells. The demonstration of T-cell-associated chromatin features in primary APL blasts has implications for the origin of APL that may arise in more primitive progenitors than previously considered to be the case.
DOI：
10.1073/pnas.93.23.13158
发表时间：
1996-11-12
影响因子：
11.1
作者：
Cheng, T;Shen, HM;Scadden, DT
通讯作者：
Scadden, DT
急性早幼粒细胞白血病患者 CD2 表达与 PML 基因断点的相关性
影响因子：
20.3
作者：
Claxton,DF;Reading,CL;Nagarajan,L;Tsujimoto,Y;Andersson,BS;Estey,E;Cork,A;Huh,YO;Trujillo,J;Deisseroth,AB
通讯作者：
Deisseroth,AB