Propagation of mechanically induced intercellular calcium waves via gap junctions and ATP receptors in rat liver epithelial cells
Propagation of mechanically induced intercellular calcium waves via gap junctions and ATP receptors in rat liver epithelial cells
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DOI：
10.1006/excr.1996.3409
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发表时间：
1997-02-01
影响因子：
3.7
通讯作者：
deFeijter, AW
中科院分区：
文献类型：
--
作者：
Frame, MK;deFeijter, AW
用机械刺激的方法在大鼠肝上皮细胞上启动钙波，以确定缝隙连接细胞间通讯(GJIC)在多大程度上参与了钙离子与相邻细胞的通讯，并评估了这些细胞之间可能存在的其他C2+信号通路。在GJIC高度表达的WB-F344细胞和GJIC缺乏的WB-AB1细胞中，机械刺激单个细胞都会诱发一个钙波，它从刺激点穿过细胞边界，直接或间接地传播到与被刺激细胞接触的邻近细胞。此外，钙波被传递到附近的孤立细胞，这些细胞与受刺激的细胞没有直接或间接的接触。用18β-甘草次酸处理细胞，这种化合物已被证明可以阻断GJIC，但对钙波的传播没有显著影响。相反，P-2-嘌呤受体抑制剂苏拉明可显著降低WB-F344细胞中钙波的传播速度和程度，并完全阻断其在WB-AB1细胞中的传播。苏拉明和甘草次酸联合作用可完全阻断机械诱导的两种细胞的钙波。这些研究表明，机械诱导的大鼠肝上皮细胞损伤至少通过两条途径启动信号传递，包括通过缝隙连接的细胞间通讯和通过ATP激活的嘌呤能受体的细胞外通讯。(C)1997年学术出版社。
Mechanical stimulation was used to initiate Ca2+ waves in rat liver epithelial cells in order to ascertain the degree to which gap junctional intercellular communication (GJIC) is involved in communication of Ca2+ to adjacent cells and to assess alternative C2+ signaling pathways that may be present between these cells. In both WB-F344 cells, which show a high degree of GJIC, and WB-aB1 cells, which are GJIC deficient, mechanical stimulation of a single cell induced a Ca2+ wave which propagated away from the point of stimulation, across cell borders, to neighboring cells directly or indirectly in contact with the stimulated cell. In addition, the Ca2+ wave was transmitted to nearby isolated cells that exhibited no direct or indirect contact with the stimulated cell. Treatment of cells with 18 beta-glycyrrhetinic acid, a compound that has been shown to block GJIC, did not significantly affect propagation of the Ca2+ wave. In contrast, treatment with suramin, a P-2-purinergic receptor inhibitor, significantly reduced both the rate and the extent of Ca2+ wave propagation in WB-F344 cells and completely blocked its propagation in WB-aB1 cells. Cotreatment with suramin and glycyrrhetinic acid was found to completely block the mechanically induced Ca2+ wave in both cell lines. These studies indicate that mechanically induced cell injury in rat liver epithelial cells initiates signaling through at least two pathways, involving intercellular communication via gap junctions and extracellular communication via ATP activation of purinergic receptors. (C) 1997 Academic Press.