Non-contact quantification of laser micro-impulse in water by atomic force microscopy and its application for biomechanics
Non-contact quantification of laser micro-impulse in water by atomic force microscopy and its application for biomechanics
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原子力显微镜对水中激光微脉冲的非接触量化及其在生物力学中的应用
DOI：
10.1117/12.903239
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发表时间：
2011
影响因子：
9.1
通讯作者：
Y. Hosokawa
中科院分区：
文献类型：
--
作者：
Y. Hosokawa
我们研制了一套飞秒激光诱导冲击力的局域力测量系统，这种冲击力是由高数值孔径物镜聚焦飞秒激光入水时产生的冲击波和应力波引起的。在该系统中，通过原子力显微镜(AFM)的悬臂梁的弯曲运动来检测微米范围内的力。在此，我们计算了飞秒激光聚焦在悬臂梁附近的水中，在冲击力作用下，原子力显微镜悬臂梁的弯曲运动。根据计算结果，提出了一种估算激光焦点处冲击力总和的方法，并将其应用于细胞间黏附强度的估算。
We developed a local force measurement system of a femtosecond laser-induced impulsive force, which is due to shock and stress waves generated by focusing an intense femtosecond laser into water with a highly numerical aperture objective lens. In this system, the force localized in micron-sized region was detected by bending movement of a cantilever of atomic force microscope (AFM). Here we calculated the bending movement of the AFM cantilever when the femtosecond laser is focused in water at the vicinity of the cantilever and the impulsive force is loaded on the cantilever. From the result, a method to estimate the total of the impulsive force at the laser focal point was suggested and applied to estimate intercellular adhesion strength.