Cytoprotective Effect of Lignans from Forsythia suspensa against Peroxynitrite-induced LLC-PK1 Cell Damage
Cytoprotective Effect of Lignans from Forsythia suspensa against Peroxynitrite-induced LLC-PK1 Cell Damage
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DOI：
10.1002/ptr.2834
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发表时间：
2009-07-01
影响因子：
7.2
通讯作者：
Cui, Jian
中科院分区：
文献类型：
--
作者：
Piao, Xiang Lan;Cho, Eun Jung;Cui, Jian
越来越多的证据表明，过氧亚硝酸根（ONOO-）与多种疾病的发病机制密切相关。作为一种旨在预防ONOO-介导的毒性的药理学策略，连翘的保护活性（Thunb.）Vahl（木犀科）对ONOO-诱导的细胞损伤进行了研究，并确定了其活性成分。在生物活性导向的分级分离其二氯甲烷馏分后，分离出两种四氢呋喃木脂素，即phillygenin和8-hydroxypinopinophinol。使用肾上皮细胞LLC-PK 1评价这些木脂素对ONOO诱导的细胞死亡的保护作用。Phillygenin和8-hydroxypinopinsulinol显著减轻由ONOO-产生剂3-morpholinosydnonimine（SIN-1）引起的细胞损伤.苯基部分上的羟基取代基可能有助于这些木脂素的抗氧化活性。这些结果表明，phillygenin和8-hydroxypinocyanol可能是有用的治疗或预防应用在治疗ONOO-相关疾病。版权所有（C）2009约翰威利父子有限公司
There is mounting evidence that peroxynitrite (ONOO-) is closely related to the pathogenesis of various diseases. As a pharmacological strategy aimed at preventing ONOO--mediated toxicity, the protective activity of Forsythia suspensa (Thunb.) Vahl (Oleaceae) against ONOO--induced cellular damage was investigated and its active components identified. After bioactivity-guided fractionation of its methylene chloride fraction, two tetrahydrofurofuran lignans were isolated, namely phillygenin and 8-hydroxypinoresinol. The protective effects of these lignans against ONOO--induced cell death were evaluated using renal epithelial cell LLC-PK1. Phillygenin and 8-hydroxypinoresinol significantly reduced the cell injury by 3-morpholinosydnonimine (SIN-1), a ONOO- generator. The hydroxy substituents on the phenyl moieties may contribute to the antioxidant activities of these lignans. These results suggest that phillygenin and 8-hydroxypinoresinol may be useful for the therapeutic or preventive applications in treating ONOO--related diseases. Copyright (C) 2009 John Wiley & Sons, Ltd.