Effect of probiotics on pro-inflammatory cytokines and NF-κB activation in ulcerative colitis
Effect of probiotics on pro-inflammatory cytokines and NF-κB activation in ulcerative colitis
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DOI：
10.3748/wjg.v16.i33.4145
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发表时间：
2010-09-07
影响因子：
4.3
通讯作者：
El-Bedewy, Mohamed M.
中科院分区：
文献类型：
--
作者：
Hegazy, Sahar K.;El-Bedewy, Mohamed M.
目的：探讨益生菌对溃疡性结肠炎（UC）患者的治疗作用及其对溃疡性结肠炎患者炎症介质和核因子(NF)- κ B活化的影响。方法：将30例轻中度UC患者随机分为两组：柳氮磺胺吡啶组，给予柳氮磺胺吡啶2400 mg/d；益生菌组给予磺胺氮磺胺2400 mg/d加益生菌治疗。在使用益生菌（德尔布鲁氏乳杆菌和发酵乳杆菌）治疗8周前后对患者进行调查。采用紫外分光光度法检测小鼠结肠髓过氧化物酶（MPO）活性，ELISA法检测小鼠结肠白细胞介素(IL)-6含量，ELISA法检测小鼠粪便钙保护蛋白含量，免疫组化法和逆转录聚合酶链反应法分别检测小鼠结肠组织nf - κ B p65和肿瘤坏死因子(TNF)- α蛋白表达。结果：在研究开始时，苏木精和伊红染色显示UC患者结肠粘膜损伤和炎症，结肠MPO活性、粪便钙保护蛋白以及结肠tnf - α和NF-kappa B p65蛋白表达增加。与磺胺氮嗪组和对照组相比，使用益生菌8周可显著降低结肠IL-6浓度、tnf - α和NF-kappa B p65的表达、白细胞募集、结肠MPO活性和粪便钙保护蛋白水平（P < 0.05），从而显著改善炎症。结论：口服益生菌制剂有助于维持UC的缓解和预防复发。(C) 2010年白石登。版权所有。
AIM: To demonstrate the therapeutic effect of probiotics in patients with ulcerative colitis (UC), and their effect on inflammatory mediators and nuclear factor (NF)-kappa B activation in these patients.METHODS: Thirty patients with mild to moderate UC were randomly classified into two groups: sulfasalazine group, who received sulfasalazine 2400 mg/d; and probiotic group, who received sulfasalazine 2400 mg/d with probiotic. The patients were investigated before and after 8 wk of treatment with probiotic (Lactobacillus delbruekii and Lactobacillus fermentum). Colonic activity of myeloperoxidase (MPO) was assayed with UV spectrophotometry, the colonic content of interleukin (IL)-6 was determined by enzyme-linked immunosorbent assay (ELISA), fecal calprotectin was determined by ELISA, and expression of NF-kappa B p65 and tumor necrosis factor (TNF)-alpha proteins in colonic tissue was identified by immunohistochemistry and reverse transcription polymerase chain reaction, respectively.RESULTS: At the start of the study, colonic mucosal injury and inflammation were demonstrated in UC patients by hematoxylin and eosin staining, and an increase in colonic MPO activity, fecal calprotectin, and expression of colonic TNF-alpha and NF-kappa B p65 proteins. The use of probiotic for 8 wk significantly ameliorated the inflammation by decreasing the colonic concentration of IL-6, expression of TNF-alpha and NF-kappa B p65, leukocyte recruitment, as demonstrated by a decrease in colonic MPO activity, and the level of fecal calprotectin compared to sulfasalazine group and the control group (P < 0.05).CONCLUSION: Oral supplementation with probiotics could be helpful in maintaining remission and preventing relapse of UC. (C) 2010 Baishideng. All rights reserved.