The bioinformatics of viral metagenomics
The bioinformatics of viral metagenomics
复制标题
病毒宏基因组学的生物信息学
DOI：
--
复制
发表时间：
2013
期刊：
影响因子：
--
通讯作者：
E. Bongcam
中科院分区：
文献类型：
--
作者：
Martin Norling;O. Karlsson;E. Bongcam
宏基因组学方法为兽医和公共卫生科学提供了新的和改进的诊断工具的希望，这些工具具有前所未有的能力，可以检测临床样本中大量已知和未知的病毒。成功的宏基因组学是基于三个主要活动，其中每一个都必须是一致和可靠的方法是有用的，这些是(1)湿实验室制备，(2)测序，(3)结果的生物信息学分析。这项工作的目的是深入了解使用宏基因组学方法检测新出现的病毒、监测野生动物已知病原体的可行性，以及从兽医的角度提供一种工具，在疫情暴发的情况下快速表征病毒病原体。
Metagenomic methods provide the veterinary and public health sciences with the promise of new and improved diagnostic tools with unprecedented ability to detect a plethora of known and unknown viromes in clinical samples. Successful metagenomics is based on three main activities where each one must be consistent and reliable for the method to be useful, these are (1) wet-lab preparation, (2) sequencing, and (3) bioinformatics analysis of the results. The aim of the work is to provide insight into the feasibility of using the metagenomics approach for detection of emerging viruses, monitoring wildlife for known pathogens as well as providing a tool for rapid characterization of viral pathogens in outbreak situations from a veterinary standpoint.