Identification of immunogenic epitopes of the 170-kDa subunit adhesin of Entamoeba histolytica in patients with invasive amebiasis.
Identification of immunogenic epitopes of the 170-kDa subunit adhesin of Entamoeba histolytica in patients with invasive amebiasis.
复制标题
侵袭性阿米巴病患者溶组织内阿米巴 170 kDa 亚基粘附素免疫原性表位的鉴定。
DOI：
10.1111/j.1550-7408.1995.tb05920.x
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发表时间：
1995
期刊：
影响因子：
--
通讯作者：
Calderon,J
中科院分区：
文献类型：
--
作者：
Velazquez,C;Valette,I;Cruz,M;Labra,ML;Montes,J;StanleyJr,SL;Calderon,J
溶组织内阿米巴可引起阿米巴痢疾（AD）和肝脓肿（ALA）。对阿米巴病的保护性免疫知之甚少，这一领域的研究由于缺乏确定的阿米巴抗原而变得复杂。我们检测了AD和ALA患者外周血单个核细胞（PBMC）对含有170 kDa粘附素的重组蛋白的增殖反应。histolytica（170CR），以及两种合成肽（1和2），其衍生自预测含有T细胞表位的170 kDa序列。大量患有AD和ALA的患者具有增殖为170CR分子的PBMC，并且几个患有ALA和AD的个体具有识别一种或两种肽的T细胞。首先，来自阿米巴病非流行地区的个体对170CR蛋白或两种肽均无应答。在抗体应答方面，15例ALA患者中有9例显示对170CR蛋白的抗体。这些患者具有针对肽2的抗体。我们鉴定了来自170 kDa粘附素的肽，其可能包含一些侵袭性阿米巴病患者识别的T和B细胞表位。这些肽在研究阿米巴病的免疫应答中可能是有价值的试剂。
Entamoeba histolyticacauses amebic dysentery (AD) and liver abscess (ALA). Little is known about protective immunity to amebiasis, and studies in this area have been complicated by the paucity of defined ameba antigens. We examined the proliferative responses of peripheral blood mononuclear cells (PBMC) from patients with AD and ALA to a recombinant protein containing a portion of the 170 kDa adhesin ofE. histolytica(170CR), and to two synthetic peptides (1 and 2) derived from the 170 kDa sequence that were predicted to contain T cell epitopes. A significant number of patients with AD and ALA had PBMC that proliferated to 170CR molecule, and several individuals with ALA and AD had T cells that recognized one or both peptides. Contrarily, individuals from a non‐endemic region for amebiasis did not respond to 170CR protein, or to both peptides. In regard to antibody response, nine of fifteen patients with ALA showed antibodies to 170CR protein. These same patients had antibodies to peptide 2. We identified peptides from 170‐kDa adhesin that may contain both T and B cell epitopes recognized by some patients with invasive amebiasis. These peptides may be valuable reagents in studies of the immune response to amebiasis.