CLONING, EXPRESSION, AND ALTERNATIVE SPLICING OF THE RECEPTOR FOR ANTI-MULLERIAN HORMONE
CLONING, EXPRESSION, AND ALTERNATIVE SPLICING OF THE RECEPTOR FOR ANTI-MULLERIAN HORMONE
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DOI：
10.1210/me.8.8.1006
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发表时间：
1994-08-01
影响因子：
--
通讯作者：
CATE, R
中科院分区：
文献类型：
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作者：
DICLEMENTE, N;WILSON, C;CATE, R
抗苗勒管激素（Anti-Mullerian hormone），也称为苗勒管抑制物质或因子，是属于转化生长因子-β超家族的糖蛋白二聚体，由未成熟的支持细胞和出生后的颗粒细胞合成。抗苗勒管激素通过诱导男性胎儿苗勒管退化在性别分化中起关键作用。它也是导致牛游离马胎中胎儿卵巢发育迟缓和雄性化的原因，并可能参与控制出生后卵巢中卵泡成熟。使用一个简并探针的转化生长因子β受体超家族的一个共识区，以筛选从兔胎儿卵巢的互补DNA文库，我们克隆了一个互补的DNA编码的跨膜丝氨酸/苏氨酸激酶，这是胎儿苗勒管周围表达，在胎儿和成人颗粒细胞，并在未成熟的支持细胞。两个转录本，产生的选择性剪接的外显子编码的N-末端61个氨基酸的结构域，强烈表达在抗苗勒管激素靶器官和支持细胞。较长的569个氨基酸的同种型在COS细胞中瞬时表达时结合抗苗勒管激素，并被认为编码其功能性受体。
Anti-Mullerian hormone, also called Mullerian-inhibiting substance or factor, is a glycoprotein dimer belonging to the transforming growth factor-beta superfamily and synthesized by immature Sertoli cells and postnatal granulosa cells. Anti-Mullerian hormone plays a key role in sex differentiation by inducing the regression of Mullerian ducts in the male fetus. It is also responsible for the stunting and masculinization of fetal ovaries in bovine freemartin fetuses and may be involved in the control of follicular maturation in the postnatal ovary. Using a degenerate probe for a consensus region of the transforming growth factor-beta receptor superfamily to screen a complementary DNA library from rabbit fetal ovaries, we cloned a complementary DNA coding for a transmembrane serine/threonine kinase, which is expressed around the fetal Mullerian duct, in fetal and adult granulosa cells, and in immature Sertoli cells. Two transcripts, generated by alternative splicing of an exon coding for an N-terminal 61-amino acid domain, are strongly expressed in anti-Mullerian hormone target organs and Sertoli cells. The longer, 569-amino acid, isoform binds anti-Mullerian hormone when transiently expressed in COS cells and is believed to encode its functional receptor.