Establishment of surrogate broodstock technology in Scombridae species by germ cell transplantation
Establishment of surrogate broodstock technology in Scombridae species by germ cell transplantation
复制标题
鲭科生殖细胞移植替代亲鱼技术的建立
DOI：
10.1111/are.15791
复制
发表时间：
2022
影响因子：
2
通讯作者：
Reoto Tani,Ryosuke Yazawa,Shigeharu Kamio,Wataru Kawamura,Tetsuro Morita,Yutaka Takeuchi,Goro Yoshizaki
中科院分区：
文献类型：
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作者：
Reoto Tani,Ryosuke Yazawa,Shigeharu Kamio,Wataru Kawamura,Tetsuro Morita,Yutaka Takeuchi,Goro Yoshizaki
本研究探讨了以雌性蓝鲭鱼（Scomber australasicus）和雄性鲐鱼（s.a japonicus）杂交产生的无生殖细胞杂交种作为受体的可行性，以开发鲭鱼替代亲鱼技术，这是一个备受关注的新的养殖目标。从鲐鱼的睾丸中分离供体睾丸细胞，移植到500只杂交鲭鱼幼虫的腹膜腔中。在42名存活到1岁的男性受体中，35名成熟并产生了来自捐赠者的鲐鱼精子。其中22例只产生了供体的精子，没有检测到受体自己的精子。6只存活到1岁的雌性中有2只产了供体来源的卵子，2只成熟的雌性中有1只产了供体来源的卵子。值得注意的是，当成熟的受体被安置在一个单独的鱼缸中时，一些自然交配，并且成功地获得了由供体来源的鲐鱼卵和精子受精而产生的正常幼体。虽然该方法的效率有待进一步提高，但杂交鲭鱼可以作为鲭鱼品种育种的替代品。该技术有望为鲭鱼的养殖做出重大贡献。
In this study, we examined the feasibility of germ cell‐less hybrids, produced by a cross between female blue mackerel (Scomber australasicus) and male chub mackerel (S.japonicus), as recipients in order to develop a surrogate broodstock technology for mackerels, which is attracting attention as a new aquaculture target. Donor testicular cells, isolated from the testes of a chub mackerel, were transplanted into the peritoneal cavity of 500 hybrid mackerel larvae. Of the 42 male recipients that survived to 1 year of age, 35 matured and produced donor‐derived chub mackerel sperm. Amongst them, 22 produced only donor‐derived sperm, with none of the recipient's own sperm detected. Two of the six females that survived to 1‐year‐old produced donor‐derived eggs, and one of two matured females produced only donor‐derived eggs. Notably, when the mature recipients were housed in a single fish tank, some spontaneously mated, and normal hatchlings arising from fertilization of donor‐derived chub mackerel eggs and sperm were successfully obtained. Although the efficiency of this method needs to be improved in the future, hybrid mackerel can be used as surrogates for mackerel species breeding. This technology is expected to make a significant contribution to the breeding of mackerels.