INTERACTIONS OF NEUTRAL AMINO ACIDS WITH HUMAN SERUM ALBUMIN AND GAMMA-GLOBULIN
INTERACTIONS OF NEUTRAL AMINO ACIDS WITH HUMAN SERUM ALBUMIN AND GAMMA-GLOBULIN
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DOI：
10.1021/ja01623a052
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发表时间：
1955-01-01
影响因子：
15
通讯作者：
COMMERFORD, SL
中科院分区：
文献类型：
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作者：
HUNTER, MJ;COMMERFORD, SL
为了发现中性氨基酸是否在中性pH下与蛋白质结合，将人血清白蛋白和人丙种球蛋白用十倍过量的C14标记的甘氨酸、亮氨酸或苯丙氨酸进行透析，并对蛋白质溶液和透析液进行放射性测量。在上述条件下，不能证明蛋白质与氨基酸的结合。开发了一种改进的技术，可以将含有 C14 标记氨基酸的蛋白质溶液移至平板上，干燥并计数。
In order to discover whether or not neutral amino acids were bound by proteins at neutral pH, human serum albumin and human gamma-globulin were dialyzed against a tenfold excess of C14-labeled glycine, leucine or phenylalanine, and radioactivity measurements performed on the protein solution and the dialysate. Under the above conditions no binding of the amino acids by the proteins could be demonstrated. An improved technique was developed whereby protein solutions containing C14-labeled amino acids could be pipetted onto the planchets, dried and counted.