Dentin remineralization in acidic solution without initial calcium phosphate ions via poly(amido amine) and calcium phosphate nanocomposites after fluid challenges.
Dentin remineralization in acidic solution without initial calcium phosphate ions via poly(amido amine) and calcium phosphate nanocomposites after fluid challenges.
复制标题
在液体挑战后，通过聚（酰氨基胺）和磷酸钙纳米复合材料在酸性溶液中进行牙本质再矿化，无需初始磷酸钙离子。
DOI：
10.1007/s00784-021-04124-y
复制
发表时间：
--
影响因子：
3.4
通讯作者：
Xu Hockin H.K.
中科院分区：
文献类型：
--
作者：
Liang Kunneng;Gao Yuan;Tao Siying;Weir Michael D;Zhou Chenchen;Li Jiyao;Xu Hockin H.K.
目的先前的研究表明，聚酰胺胺(PAMAM)与无定形磷酸钙纳米颗粒(NACP)的可充电复合材料在酸性溶液中诱导牙本质再矿化，模拟口干患者的口腔状况。然而，口腔内频繁的液体挑战可能会降低再矿化能力。因此，本研究的目的是首次研究酸性溶液中PAMAM- + 纳米复合材料在酸性溶液中对牙本质的再矿化效果。将脱矿后的牙本质分为4组：(1)对照牙本质；(2)PAMAM涂层牙本质；(3)NACP复合体；(4)PAMAM- + 修复牙本质。将PAMAM涂层的牙本质在磷酸盐缓冲液中摇动77天，以将PAMAM从牙本质中解吸出来。在无初始钙磷离子的pH为4的乳酸中处理42d。结果液体激发77d后，PAMAM不能阻止牙本质在乳酸中脱矿。充电后的NaCP纳米复合材料将pH值提高到6.5以上，每天分别重新释放超过6.0kmo1/L的Ca和P离子，抑制了进一步的脱矿。相反，PAMAM + NACP复合体可诱导牙本质再矿化，使牙本质显微硬度恢复至0.54 ± 0.04 Gpa，接近正常牙本质的再矿化水平(P= 0.426，P>； 0.05)。结论PAMAM + NACP复合体可在酸性溶液中实现牙本质再矿化，无初始钙磷离子，即使在严重的液体条件下也是如此。临床意义新型PAMAM + NACP具有强大而持久的再矿化能力，即使在口腔干燥的情况下也能抑制继发性龋病。
ObjectivesA previous study showed that the combination of poly(amido amine) (PAMAM) and rechargeable composites with nanoparticles of amorphous calcium phosphate (NACP) induced dentin remineralization in an acidic solution with no initial calcium (Ca) and phosphate (P) ions, mimicking the oral condition of individuals with dry mouths. However, the frequent fluid challenge in the oral cavity may decrease the remineralization capacity. Therefore, the objective of the present study was to investigate the remineralization efficacy on dentin in an acid solution via PAMAM + NACP after fluid challenges for the first time.MethodsThe NACP nanocomposite was stored in a pH 4 solution for 77 days to exhaust its Ca and P ions and then recharged. Demineralized dentin samples were divided into four groups: (1) control dentin, (2) dentin coated with PAMAM, (3) dentin with recharged NACP composite, and (4) dentin with PAMAM + recharged NACP. PAMAM-coated dentin was shaken in phosphate-buffered saline for 77 days to desorb PAMAM from dentin. Samples were treated in pH 4 lactic acid with no initial Ca and P ions for 42 days.ResultsAfter 77 days of fluid challenge, PAMAM failed to prevent dentin demineralization in lactic acid. The recharged NACP nanocomposite raised the pH to above 6.5 and re-released more than 6.0 and 4.0 mmol/L Ca and P ions daily, respectively, which inhibited further demineralization. In contrast, the PAMAM + NACP combined method induced great dentin remineralization and restored the dentin microhardness to 0.54 ± 0.04 GPa, which approached that of sound dentin (P= 0.426,P> 0.05).ConclusionsThe PAMAM + NACP combination achieved dentin remineralization in an acid solution with no initial Ca and P ions, even after severe fluid challenges.Clinical relevanceThe novel PAMAM + NACP has a strong and sustained remineralization capability to inhibit secondary caries, even for individuals with dry mouths.