PROGRESSION OF THE CELL-CYCLE THROUGH MITOSIS LEADS TO ABORTION OF NASCENT TRANSCRIPTS
PROGRESSION OF THE CELL-CYCLE THROUGH MITOSIS LEADS TO ABORTION OF NASCENT TRANSCRIPTS
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DOI：
10.1016/0092-8674(91)90182-x
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发表时间：
1991-10-18
期刊：
影响因子：
64.5
通讯作者：
OFARRELL, PH
中科院分区：
文献类型：
--
作者：
SHERMOEN, AW;OFARRELL, PH
通过原位杂交检测果蝇Ubx基因的新生转录本。表达开始后，RNA聚合酶（1.4 kb/min）在基因上的进展被可视化为来自转录单位内不同位置的杂交信号的连续出现。新生转录本在有丝分裂时消失。杂交信号在下一个细胞周期中再次出现，首先用5'探针，随后，在与转录速率一致的延迟后，用3'探针。在细胞在间期被阻断的突变胚胎的表达细胞中连续观察到新生转录物。我们的结论是，通过有丝分裂的进展导致流产的新生成绩单，并建议定期流产的转录有助于调节大基因的表达。
Nascent transcripts of the Drosophila Ubx gene were detected by in situ hybridization. Following onset of expression, the progress of RNA polymerase (1.4 kb/min) across the gene was visualized as the successive appearance of hybridization signals from different positions within the transcription unit. Nascent transcripts disappeared at mitosis. Hybridization signals reappeared in the next cell cycle first with a 5' probe, and later, following a delay consistent with the transcription rate, with a 3' probe. Nascent transcripts were observed continuously in expressing cells of a mutant embryo in which cells are blocked in interphase. We conclude that progression through mitosis causes abortion of nascent transcripts and suggest that periodic abortion of transcription contributes to regulation of expression of large genes.