Practical reconstruction method for bioluminescence tomography
Practical reconstruction method for bioluminescence tomography
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DOI：
10.1364/opex.13.006756
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发表时间：
2005-09-05
期刊：
影响因子：
3.8
通讯作者：
Cong, A
中科院分区：
文献类型：
--
作者：
Cong, WX;Wang, G;Cong, A
生物发光断层扫描 (BLT) 用于定位和量化小型活体动物的生物发光源。通过将生物发光成像推进到断层扫描框架，它有助于诊断疾病、监测治疗并促进药物开发。在本文中，我们通过使用基于生物组织中光子传播的扩散近似的有限元方法，在测量的表面光子密度和未知的生物发光源分布之间建立了直接的线性关系。我们开发了一种新颖的重建算法来恢复源分布。该算法结合先验知识来定义允许的源区域，以增强数值稳定性和效率。数值小鼠胸部模型的模拟证明了所提出的 BLT 算法的可行性，并揭示了其在源位置、密度和抗噪声鲁棒性方面的性能。最后，进行 BLT 实验来确定物理小鼠胸部模型中两个光源的位置和功率。 (c) 2005 年美国光学学会
Bioluminescence tomography (BLT) is used to localize and quantify bioluminescent sources in a small living animal. By advancing bioluminescent imaging to a tomographic framework, it helps to diagnose diseases, monitor therapies and facilitate drug development. In this paper, we establish a direct linear relationship between measured surface photon density and an unknown bioluminescence source distribution by using a finite- element method based on the diffusion approximation to the photon propagation in biological tissue. We develop a novel reconstruction algorithm to recover the source distribution. This algorithm incorporates a priori knowledge to define the permissible source region in order to enhance numerical stability and efficiency. Simulations with a numerical mouse chest phantom demonstrate the feasibility of the proposed BLT algorithm and reveal its performance in terms of source location, density, and robustness against noise. Lastly, BLT experiments are performed to identify the location and power of two light sources in a physical mouse chest phantom. (c) 2005 Optical Society of America