Empoasca leafhoppers attack wild tobacco plants in a jasmonate-dependent manner and identify jasmonate mutants in natural populations
Empoasca leafhoppers attack wild tobacco plants in a jasmonate-dependent manner and identify jasmonate mutants in natural populations
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DOI：
10.1073/pnas.1200363109
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发表时间：
2012-06-12
影响因子：
11.1
通讯作者：
Baldwin, Ian T.
中科院分区：
文献类型：
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作者：
Kallenbach, Mario;Bonaventure, Gustavo;Baldwin, Ian T.
植食性昆虫对寄主植物的选择对于它们的生存和繁殖至关重要。这种选择涉及对潜在供食植物的各种物理和化学特征的复杂行为反应。对于半翅目昆虫，这些行为反应涉及一系列步骤，包括轻拍阴唇和使用刺穿的口器进行探测。这些最初的探测和喂养尝试还会引起植物激素（例如茉莉酸（JA））及其介导的诱导防御代谢物的快速积累。当通过沉默 JA 生物合成途径的初始关键步骤而使烟草植物缺乏 JA 时，它们在自然界中会受到小绿叶蝉属半翅目叶蝉的严重攻击。通过生产在 JA 生物合成和感知的多个步骤以及植物的 JA 诱导杀虫防御的生物合成的多个步骤中沉默的 N. attenuata 植物，我们证明了小绿叶蝉在自然界和温室中取食的植物的选择与主要杀虫分子的积累无关。此外，这种选择与 Candidatus Phytoplasma spp 的存在无关。并且与植物挥发物的可检测变化无关，而是取决于植物介导 JA 信号传导的能力。我们利用这一特性并使用小绿叶蝉叶蝉来揭示 JA 积累的遗传变异以及隐藏在 N. attenuata 自然种群中的信号传导。
Choice of host plants by phytophagous insects is essential for their survival and reproduction. This choice involves complex behavioral responses to a variety of physical and chemical characteristics of potential plants for feeding. For insects of the order Hemiptera, these behavioral responses involve a series of steps including labial dabbing and probing using their piercing mouthparts. These initial probing and feeding attempts also elicit a rapid accumulation of phytohormones, such as jasmonic acid (JA), and the induced defense metabolites they mediate. When Nicotiana attenuata plants are rendered JA deficient by silencing the initial committed step of the JA biosynthesis pathway, they are severely attacked in nature by hemipteran leafhoppers of the genus Empoasca. By producing N. attenuata plants silenced in multiple steps of JA biosynthesis and perception and in the biosynthesis of the plant's three major classes of JA-inducible insecticidal defenses, we demonstrate that the choice of plants for feeding by Empoasca leafhoppers in both nature and the glasshouse is independent of the accumulation of major insecticidal molecules. Moreover, this choice is independent of the presence of Candidatus Phytoplasma spp. and is not associated with detectable changes in plant volatiles but instead depends on the plant's capacity to mediate JA signaling. We exploited this trait and used Empoasca leafhoppers to reveal genetic variation in JA accumulation and signaling hidden in N. attenuata natural populations.