NUCLEOSIDE TRIPHOSPHATE REGENERATION DECREASES THE FREQUENCY OF TRANSLATION ERRORS
NUCLEOSIDE TRIPHOSPHATE REGENERATION DECREASES THE FREQUENCY OF TRANSLATION ERRORS
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DOI：
10.1073/pnas.76.7.3174
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发表时间：
1979-01-01
影响因子：
11.1
通讯作者：
KURLAND, CG
中科院分区：
文献类型：
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作者：
JELENC, PC;KURLAND, CG
将天然存在的多胺和无机离子添加到体外蛋白质合成系统[利用大肠杆菌提取物]中，提高了翻译的程度和保真度。在这种优化的系统中，核苷三磷酸与磷酸烯醇丙酮酸和丙酮酸激酶（ATP：丙酮酸2-O-磷酸转移酶，EC 2.7.1.40）的再生进一步将错义错误频率降低到体内水平并增强翻译的程度。核苷三磷酸再生的效果主要是由于ATP和GTP与其水解产物的比率增加，并且仅轻微地由于核苷三磷酸的绝对浓度增加。
The addition of naturally occurring polyamines and inorganic ions to an in vitro protein-synthesizing system [utilizing Escherichia coli extract] improved the extent and fidelity of translation. In such an optimized system, regeneration of the nucleoside triphosphates with phosphoenolpyruvate and pyruvate kinase (ATP:pyruvate 2-O-phosphotransferase, EC 2.7.1.40) further reduced the missense error frequency to the in vivo level and enhanced the extent of translation. The effect of nucleoside triphosphate regeneration was due primarily to the increase in the ratio of ATP and GTP to their hydrolysis products and was only marginally due to the increase in the absolute concentrations of the nucleoside triphosphates.