Enhanced expression of IL-10 in contrast to IL-12B mRNA in poultry with experimental coccidiosis
Enhanced expression of IL-10 in contrast to IL-12B mRNA in poultry with experimental coccidiosis
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DOI：
10.1016/j.exppara.2012.08.017
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发表时间：
2012-11-01
影响因子：
2.1
通讯作者：
Stanilova, S. A.
中科院分区：
文献类型：
--
作者：
Haritova, A. M.;Stanilova, S. A.
IL-12和IL-10是免疫调节性细胞因子，具有拮抗(Th)1/Th2细胞因子平衡的作用，它们在天然抵抗和免疫应答之间提供了功能联系。为探讨柔嫩艾美耳球虫感染后雏鸡肠道黏膜中IL-10及其mRNA表达水平的变化，将肉鸡随机分为3组：健康对照组、未处理组和感染后6天用磺胺吡嗪钠治疗组。用IL-10和IL-12B的特异性引物和探针进行实时定量聚合酶链式反应(RT-PCR)分析。柔嫩艾美耳球虫感染后，盲肠IL-10mRNA表达显著上调，肝脏IL-10mRNA表达明显上调。磺胺吡嗪作用后，主要在盲肠组织中出现明显的下调。相反，IL-12B在所有被研究的动物组织中的表达仍然受到影响。在柔嫩艾美耳球虫攻击后，盲肠组织中IL-10mRNA的表达明显上调，这可归因于艾美耳球虫的组织嗜性。IL-12的产生是通过IL-10负性调节的，这解释了IL-12bmRNA缺乏增加的原因。磺胺治疗后，IL-10mRNA的水平得到改善并恢复到健康鸡的水平。(C)2012年Elsevier Inc.所有权利
Interleukin (IL)-12 and IL-10 are immunoregulatory cytokines with an antagonistic effect on the (Th)1/Th2 cytokine balance and they provide a functional link between innate resistance and the immune response. This investigation was conducted to determine the expression of IL-10 and mRNA levels in chickens' gut mucosa infected with Eimeria tenella and in sulfachlorpyrazine-sodium treated animals after infection.Broiler chickens were randomly allocated in three groups: healthy untreated control; untreated animals and infected, treated with sulfachlorpyrazine sodium chickens 6 days after the challenge with an E. tenella. Quantitative real time PCR analysis was performed using specific primer and probes for IL-10 and IL-12B.The expression of IL-10 mRNA was greater in the duodenum then in the caecum and the liver of chickens. E. tenella infection led to significant up-regulation of IL-10 mRNA in the caecum, followed mRNA in the liver. A significant down regulation was observed mainly in the caecum after the with sulfachlorpyrazine. In contrast, IL-12B expression in all investigated tissues remained affected in the studied groups of animals.Distinct up-regulation of IL-10 mRNA, after the challenge with E. tenella, in the caecum can be attributed to the tissue tropism of Eimeria spp. The production of IL-12 is regulated by negative through IL-10 which explains lack of increase in IL-12B mRNA. Sulfonamide treatment resulted in improvement and restoration of IL-10 mRNA to the levels observed in healthy chickens. (C) 2012 Elsevier Inc. All rights