Autophagy in normal tissues of camel (Camelus dromedaries) with focus on immunoexpression of LC3 and LC3B
Autophagy in normal tissues of camel (Camelus dromedaries) with focus on immunoexpression of LC3 and LC3B
复制标题
骆驼（单峰骆驼）正常组织中的自噬，重点关注 LC3 和 LC3B 的免疫表达
DOI：
10.1080/10520295.2018.1470728
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发表时间：
2018
影响因子：
1.6
通讯作者：
Shintani Michiko
中科院分区：
文献类型：
--
作者：
Osman Abdel-Hamid K.;Shintani Michiko
自噬是胞质蛋白聚集体和整个细胞器降解和再循环的高度调控的细胞内途径。营养饥饿刺激了自噬途径，这促使我们研究沙漠骆驼。由于食物和水的缺乏、脱水和长时间暴露在太阳辐射下，骆驼的各个器官受到生态和生理上的压力。我们研究了正常条件下自噬特异性生物标志物的免疫组织化学表达，作为后期应激个体工作的基线。自噬特异性生物标志物微管相关蛋白1轻链3 （LC3）及其裂解变体LC3B在所有检测组织的细胞质中均有强烈表达。LC3的胞质免疫反应性较弱，呈弥漫性、空泡性，而LC3B的胞质免疫反应性较强，呈点状、低水平。LC3似乎与自由或溶酶体结合的自噬体膜有关。LC3B对自噬体-溶酶体复合物及其降解的颗粒状内容物具有特异性。自噬在中枢神经系统神经元和肠道神经元件中强烈表达，提示其对神经系统具有保护作用。在淋巴结、脾脏和肠道相关淋巴组织（GALT）中细胞核碎片化的畸形免疫能力细胞中也发现了自噬标记物，这表明存在消除不需要细胞的“自杀”行为。通过LC3和LC3B的表达来检测自噬，可能在荒漠骆驼组织中参与了一个普遍的调控机制。
Autophagy is a highly regulated intracellular pathway for degradation and recycling of cytoplasmic protein aggregates and entire organelles. The autophagic pathway is stimulated by nutrient starvation, which prompted us to study the desert camel. Various organs of the camel undergo ecological and physiological stress due to food and water deprivation, dehydration and long exposure to solar radiation. We investigated the immunohistochemical expression of specific biomarkers of autophagy under normal conditions as a baseline for later work on stressed individuals. The autophagy-specific biomarkers, microtubule-associated protein1 light chain 3 (LC3), and its cleaved variant, LC3B, were strongly expressed in the cytosol of all tissues examined. The cytosolic immunoreactivity of LC3 was relatively weak, diffuse and vacuolar, while that of LC3B was stronger, punctate and at lower levels. LC3 appears to be associated with the autophagosomal membranes, either free or lysosome-bounded. LC3B is specific for the autophagosome-lysosome complexes and their degraded, granular contents. Autophagy was strongly expressed in CNS neurons and intestinal neural elements, which suggests a protective function for the nervous system. Autophagic markers also were seen in deformed immune-competent cells with fragmented nuclei in lymph nodes, spleen and gut-associated lymphoid tissue (GALT), which suggests a “suicidal” activity of eliminating unneeded cells. Autophagy, as measured by LC3 and LC3B expression, may participate in a general regulatory mechanism in tissues of the desert camel.