Bacterial protein toxins and lipids: pore formation or toxin entry into cells
Bacterial protein toxins and lipids: pore formation or toxin entry into cells
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DOI：
10.1042/bc20050082
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发表时间：
2006-11-01
影响因子：
2.7
通讯作者：
Popoff, Michel R.
中科院分区：
文献类型：
--
作者：
Geny, Blandine;Popoff, Michel R.
脂类是在细胞中起关键作用的疏水分子，尤其是膜的基本成分，而细菌毒素主要是亲水性蛋白质。所有细菌毒素首先通过识别表面受体与其靶细胞相互作用，表面受体要么是脂类，要么是脂类衍生物，或者是另一种化合物，但在脂类环境中。大多数细菌毒素是PFT(孔洞形成毒素)，它可以寡聚并插入到脂质双层中。一种常见的作用机制涉及形成β-桶结构，该结构由单个单体的单个β-发夹(S)组装而成。细胞内活性毒素的一个重要步骤是将其酶部分转移到胞浆中。一些毒素使用一种类似于PFTs的基于毛孔形成的易位机制，另一些毒素则经历一种尚不清楚的“伴侣”过程。
Lipids are hydrophobic molecules which play critical functions in cells, in particular, they are essential constituents of membranes, whereas bacterial toxins are mainly hydrophilic proteins. All bacterial toxins interact first with their target cells by recognizing a surface receptor, which is either a lipid or a lipid derivative, or another compound but in a lipid environment. Most bacterial toxins are PFTs (pore-forming toxins) which oligomerize and insert into the lipid bilayer. A common mechanism of action involves the formation of a beta-barrel structure, resulting from the assembly of individual beta-hairpin(s) from individual monomers. An essential step for intracellular active toxins is to translocate their enzymatic part into the cytosol. Some toxins use a translocation mechanism based on pore formation similar to that of PFTs, others undergo a yet unclear 'chaperone' process.