Dynamic cell stretching increases human osteoblast proliferation and CICP synthesis but decreases osteocalcin synthesis and alkaline phosphatase activity
Dynamic cell stretching increases human osteoblast proliferation and CICP synthesis but decreases osteocalcin synthesis and alkaline phosphatase activity
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DOI：
10.1016/s0021-9290(99)00171-2
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发表时间：
2000-01-01
影响因子：
2.4
通讯作者：
Claes, L
中科院分区：
文献类型：
--
作者：
Kaspar, D;Seidl, W;Claes, L
在生理负荷骨组织中发生的量级的循环应变的机械刺激后，研究了人骨源性细胞培养物的细胞活性。单层的亚汇合生长的人骨来源的细胞在矩形硅皿中拉伸，主要沿其纵轴沿着进行周期性单轴运动。在两天内施加应变，每天30分钟，频率为1 Hz，应变幅度为1000 mu应变。细胞的周期性拉伸导致人松质骨来源的成骨细胞的增殖增加（10- 48%）和羧基末端胶原I型前肽释放增加（7- 49%），而碱性磷酸酶活性和骨钙素释放分别显著降低9- 25%和5- 32%。这些结果表明，在生理幅度的周期性应变导致与基质生产相关的成骨细胞活性的增加，而那些分化的成骨细胞的特征和基质矿化相关的活动减少。(C)1999爱思唯尔科技有限公司。保留所有权利。
The cell activity of human-bone-derived cell cultures was studied after mechanical stimulation by cyclic strain at a magnitude occurring in physiologically loaded bone tissue. Monolayers of subconfluently grown human-bone-derived cells were stretched in rectangular silicone dishes with cyclic predominantly uniaxial movement along their longitudinal axes. Strain was applied over two days for 30 min per day with a frequency of 1 Hz and a strain magnitude of 1000 mu strain. Cyclic stretching of the cells resulted in an increased proliferation (10-48 %) and carboxyterminal collagen type I propeptide release (7-49 %) of human-cancellous bone-derived osteoblasts while alkaline phosphatase activity and osteocalcin release were significantly reduced by 9-25 and 5-32%, respectively. These results demonstrate that cyclic strain at physiologic magnitude leads to an increase of osteoblast activities related to matrix production while those activities which are characteristic for the differentiated osteoblast and relevant for matrix mineralization are decreased. (C) 1999 Elsevier Science Ltd. All rights reserved.