Morphological analysis of endocytosis in efferent ductules of estrogen receptor-alpha knockout male mouse.
Morphological analysis of endocytosis in efferent ductules of estrogen receptor-alpha knockout male mouse.
复制标题
雌激素受体-α敲除雄性小鼠传出小管内吞作用的形态学分析。
DOI：
10.1002/ar.1071
复制
发表时间：
2001
期刊：
影响因子：
--
通讯作者：
Hess,RA
中科院分区：
文献类型：
--
作者：
Nakai,M;Bouma,J;Nie,R;Zhou,Q;Carnes,K;Lubahn,DB;Hess,RA
雌激素受体（ER）缺乏会导致传出小管积液和扩张，这表明雌激素和 ER 在男性生殖道中的作用与小管中的液体重吸收有关。在本研究中，对野生型（WT）和雌激素受体-α 敲除（αERKO）雄性小鼠传出小管非纤毛细胞的内吞作用进行了形态学比较。 WT 传出小管内衬的上皮细胞呈高柱状，而 αERKO 的上皮细胞呈低柱状。免疫细胞化学结果显示，两种基因型的非纤毛细胞均显示硫酸化糖蛋白-2阳性反应，但αERKO中反应产物量减少。电镜显示，野生型的非纤毛细胞在顶端细胞质中具有许多用于内吞作用的细胞器，例如包被的小凹和囊泡、小管、内体、多泡体和溶酶体。这些细胞器在 αERKO 的非纤毛细胞中发育较差。形态计量分析表明，与 WT 相比，αERKO 的非纤毛细胞的内吞器官面积显着减少。使用金颗粒的示踪研究表明，WT 和 αERKO 传出小管的非纤毛细胞都能够吸收管腔内容物。这些结果表明，通过内吞作用对管腔内容物的重吸收发生在传出小管中，但在没有 ERα 的情况下，其量大大减少。 Anat Rec 263:10–18, 2001。© 2001 Wiley-Liss, Inc.
Lack of estrogen receptor (ER) results in fluid accumulation and dilation of the efferent ductules, suggesting that the role of estrogen and ER in the male reproductive tract is related to fluid reabsorption in the ductules. In the present study, endocytosis of the nonciliated cells of the efferent ductules was compared morphologically between wild type (WT) and estrogen receptor‐α knockout (αERKO) male mice. The epithelial cells lining the WT efferent ductules were tall columnar in shape, whereas those of the αERKO were low columnar. Immunocytochemically, the nonciliated cells of both genotypes showed positive reactions of sulfated glycoprotein‐2, but the reaction products were reduced in amount in the αERKO. Electron microscopy revealed that the nonciliated cells of the WT had numerous organelles for endocytosis such as coated pits and vesicles, tubules, endosomes, multivesicular bodies and lysosomes in the apical cytoplasm. These organelles were less developed in the nonciliated cells of the αERKO. Morphometric analysis indicated that there was a significant reduction in area of endocytotic apparatus in the nonciliated cells of the αERKO compared with that of the WT. A tracer study using gold particles demonstrated that the nonciliated cells of both WT and αERKO efferent ductules were capable of taking up luminal contents. These results suggest that reabsorption of the luminal contents via endocytosis takes place in the efferent ductules but is greatly reduced in amount in the absence of ERα. Anat Rec 263:10–18, 2001. © 2001 Wiley‐Liss, Inc.