Methylprednisolone and isoproterenol inhibit airway smooth muscle proliferation by separate and additive mechanisms.
Methylprednisolone and isoproterenol inhibit airway smooth muscle proliferation by separate and additive mechanisms.
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甲基泼尼松龙和异丙肾上腺素通过单独和相加的机制抑制气道平滑肌增殖。
DOI：
10.1016/0024-3205(96)00259-7
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发表时间：
1996
期刊：
影响因子：
6.1
通讯作者：
Noveral,JP
中科院分区：
文献类型：
--
作者：
Schramm,CM;Omlor,GJ;Quinn,LM;Noveral,JP
为了确定糖皮质激素和β-肾上腺素受体激动剂是否独立作用以抑制气道平滑肌（ASM）增殖，本研究研究了甲泼尼龙（MP）和异丙肾上腺素（ISO）单独使用和联合使用对白三烯D4诱导的ASM增殖的影响。MP和ISO对未受刺激的ASM细胞生长没有影响。相比之下，MP和ISO表现出对LTD 4诱导的增殖的剂量依赖性抑制，并且MP和ISO的组合的抑制作用是相加的。竞争性cAMP受体拮抗剂Rp-cAMPS可解除ISO对细胞的抑制作用，但对MP的抑制作用无影响。在暴露于ISO和MP的细胞中，Rp-cAMPS将生长抑制减弱至单独MP所达到的水平。因此，这些发现表明糖皮质激素和β-肾上腺素能激动剂抑制LTD 4诱导的ASM增殖，并且它们的抑制作用是由不同的信号传导途径介导的。
To determine whether glucocorticoids and β-adrenoceptor agonists act independently to inhibit airway smooth muscle (ASM) proliferation, the present study investigated the effects of methylprednisolone (MP) and isoproterenol (ISO) alone and in combination on leukotriene D4-induced ASM proliferation. MP and ISO had no effect on unstimulated ASM cell growth. In contrast, MP and ISO demonstrated dose-dependent inhibition of LTD4-induced proliferation, and the inhibitory effect was additive for combinations of MP and ISO. The competitive cAMP receptor antagonist, Rp-cAMPS, ablated the ISO-induced inhibition but had no affect on the inhibitory response to MP. In cells exposed to both ISO and MP, Rp-cAMPS attenuated the growth inhibition to levels achieved by MP alone. Accordingly, these findings demonstrate that glucocorticoids and β-adrenergic agonists inhibit LTD4-induced ASM proliferation, and that their inhibitory effects are mediated by different signaling pathways.