The effect of nutrient deposition on bacterial communities in Arctic tundra soil
The effect of nutrient deposition on bacterial communities in Arctic tundra soil
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DOI：
10.1111/j.1462-2920.2010.02189.x
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发表时间：
2010-07-01
影响因子：
5.1
通讯作者：
Schuur, Edward A. G.
中科院分区：
文献类型：
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作者：
Campbell, Barbara J.;Polson, Shawn W.;Schuur, Edward A. G.
由于气候变暖和活性氮沉积增加，预计高纬度生态系统的微生物群落在下个世纪将经历快速变化，这些变化可能会影响微生物群落的结构和功能。在阿拉斯加布鲁克斯山脉北坡的潮湿酸性苔原（MAT）土壤中，长期添加养分后曾观察到碳和氮的大量损失。为了分析微生物群落在这些损失中的作用，我们利用 16S rRNA 基因标签焦磷酸测序结合群落水平的生理分析来描述四组配对对照和施肥 MAT 土壤样品中短期和长期养分施肥后 MAT 细菌群落的变化。长期施肥小区的细菌多样性较低。酸杆菌是所有土壤中最丰富的门之一，矿物土层和有机土层之间酸杆菌亚群的分布存在明显差异，这些差异也受到施肥的影响。此外，与对照土壤相比，长期施肥样品中的α-和γ-变形菌更加丰富。被鉴定为 Dyella spp 的 Gammaproteobacteria 中的序列急剧增加。通过定量 PCR (qPCR) 在多个样品中证实了长期受精样品中存在 Xanthomonadales（黄单胞菌目）。长期施肥也与土壤中存在的微生物有关。综合数据表明，长期施肥导致微生物群落结构和功能发生显着变化，这与碳和氮可用性的变化以及地上植物群落的变化有关。
The microbial communities of high-latitude ecosystems are expected to experience rapid changes over the next century due to climate warming and increased deposition of reactive nitrogen, changes that will likely affect microbial community structure and function. In moist acidic tundra (MAT) soils on the North Slope of the Brooks Range, Alaska, substantial losses of C and N were previously observed after long-term nutrient additions. To analyse the role of microbial communities in these losses, we utilized 16S rRNA gene tag pyrosequencing coupled with community-level physiological profiling to describe changes in MAT bacterial communities after short-and long-term nutrient fertilization in four sets of paired control and fertilized MAT soil samples. Bacterial diversity was lower in long-term fertilized plots. The Acidobacteria were one of the most abundant phyla in all soils and distinct differences were noted in the distributions of Acidobacteria subgroups between mineral and organic soil layers that were also affected by fertilization. In addition, Alpha- and Gammaproteobacteria were more abundant in long-term fertilized samples compared with control soils. The dramatic increase in sequences within the Gammaproteobacteria identified as Dyella spp. (order Xanthomonadales) in the long-term fertilized samples was confirmed by quantitative PCR (qPCR) in several samples. Long-term fertilization was also correlated microbes present in the soils. The combined data indicate that long-term fertilization resulted in a significant change in microbial community structure and function linked to changes in carbon and nitrogen availability and shifts in above-ground plant communities.