Developing a cadmium resistant rice genotype with OsHIPP29 locus for limiting cadmium accumulation in the paddy crop.
Developing a cadmium resistant rice genotype with OsHIPP29 locus for limiting cadmium accumulation in the paddy crop.
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DOI：
10.1016/j.chemosphere.2020.125958
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发表时间：
2020-01
期刊：
影响因子：
8.8
通讯作者：
Bai-Qing Zhang;X. Liu;S. Feng;Ya Ning Zhao;L. Wang;J. Rono;He Li-;Z. Yang
中科院分区：
文献类型：
--
作者：
Bai-Qing Zhang;X. Liu;S. Feng;Ya Ning Zhao;L. Wang;J. Rono;He Li-;Z. Yang
农业土壤受到镉等有毒金属的广泛污染是对作物生产和人类健康的主要威胁。金属伴侣蛋白是一类独特的蛋白质，在细胞内的金属离子解毒中起着关键作用。在这项研究中，我们研究了一种名为OsHIPP29的金属伴侣蛋白在水稻植物中的生物学功能，发现OsHIPP29存在于质膜和细胞核中，并能解毒过量的Cd和Zn。oshipp29主要在营养期的芽中表达，在花期的叶鞘和小穗中表达。过量的Cd、Zn、Cu、Fe和Mn可不同程度地诱导。为了确定oshipp29在镉胁迫下介导水稻响应中的功能，我们研究了一对oshipp29突变体、RNAi系和在镉胁迫下过表达goshipp29 （OX）的转基因水稻。突变系和RNAi系在生长过程中都对Cd敏感，表现为株高和干生物量的下降。相比之下，OX系在Cd处理下表现出更好的生长。与表型一致，OX系在根和茎组织中积累的Cd较少，而oshipp29敲除导致Cd积累增加。这些结果表明，oshipp29的表达能够通过减少Cd在水稻植株中的积累来促进Cd解毒。我们的工作强调了oshipp29介导的水稻植株Cd减少的重要性，这对进一步开发基因型具有重要意义，这些基因型将最大限度地减少水稻中Cd的积累和对人类健康的环境风险。
Widespread contamination of agricultural soil with toxic metals such as cadmium (Cd) is a major threat to crop production and human health. Metallochaperones are a unique class of proteins that play pivotal roles in detoxifying metallic ions inside cells. In this study, we investigated the biological function of an uncharacterized metallochaperone termed OsHIPP29 in rice plants and showed that OsHIPP29 resides in the plasma membrane and nucleus and detoxifies excess Cd and Zn.OsHIPP29was primarily expressed in shoots during the vegetative stage and in leaf sheath and spikelet at the flowering stage. It can be differentially induced by excess Cd, Zn, Cu, Fe and Mn. To identify the function ofOsHIPP29in mediating rice response to Cd stress, we examined a pair ofOsHIPP29mutants, RNAi lines and transgenic rice overexpressingOsHIPP29(OX) under Cd stress. Both mutant and RNAi lines are sensitive to Cd in growth as reflected in decreased plant height and dry biomass. In contrast, the OX lines showed better growth under Cd exposure. Consistent with the phenotype, the OX lines accumulated less Cd in both root and shoot tissues, whereasOsHIPP29knockout led to higher accumulation of Cd. These results point out that expression ofOsHIPP29is able to contribute to Cd detoxification by reducing Cd accumulation in rice plants. Our work highlights the significance ofOsHIPP29-mediated reduced Cd in rice plants, with important implications for further developing genotypes that will minimize Cd accumulation in rice and environmental risks to human health.